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Titolo:
MONOCLONAL-ANTIBODIES TO GROUP-II DERMATOPHAGOIDES SPP ALLERGENS - MURINE IMMUNE-RESPONSE, EPITOPE ANALYSIS, AND DEVELOPMENT OF A 2-SITE ELISA
Autore:
OVSYANNIKOVA IG; VAILES LD; LI Y; HEYMANN PW; CHAPMAN MD;
Indirizzi:
UNIV VIRGINIA,CTR UVA ASTHMA & ALLERG DIS,DEPT MED,DIV ALLERGY & CLINIMMUNOL,BOX 225 CHARLOTTESVILLE VA 22908 UNIV VIRGINIA,CTR UVA ASTHMA & ALLERG DIS,DEPT MED,DIV ALLERGY & CLINIMMUNOL CHARLOTTESVILLE VA 22908 UNIV VIRGINIA,DEPT PEDIAT CHARLOTTESVILLE VA 00000
Titolo Testata:
Journal of allergy and clinical immunology
fascicolo: 3, volume: 94, anno: 1994,
parte:, 1
pagine: 537 - 546
SICI:
0091-6749(1994)94:3<537:MTGDSA>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
HOUSE-DUST MITE; DER-P-I; HUMAN IGE ANTIBODIES; F-I; ASTHMATIC-CHILDREN; PTERONYSSINUS; EXPOSURE; ANTIGEN; SENSITIZATION; FARINAE;
Keywords:
MONOCLONAL ANTIBODY; MITE ALLERGENS; ELISA; DERMATOPHAGOIDES; ASTHMA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
I.G. Ovsyannikova et al., "MONOCLONAL-ANTIBODIES TO GROUP-II DERMATOPHAGOIDES SPP ALLERGENS - MURINE IMMUNE-RESPONSE, EPITOPE ANALYSIS, AND DEVELOPMENT OF A 2-SITE ELISA", Journal of allergy and clinical immunology, 94(3), 1994, pp. 537-546

Abstract

Background: Group II allergens are a major cause of sensitization in patients allergic to mites. To facilitate the antigenic analysis of group II allergens and to develop improved methods of allergen detectionwe compared IgG anti-group II antibody responses in inbred mouse strains and raised a panel of monoclonal antibodies (mAbs). Methods: IgE antibody responses were compared by antigen-binding radioimmunoassay. Epitope specificity of the mAbs was analyzed by two-site binding assaysand by cross-inhibition radioimmunoassays. Results: Comparison of polyclonal IgG antibody responses in five BALB congenic strains showed that H-2(d) mice had poor responses, whereas H-2(b) and H-2(k) mice had strong, cross-reactive, IgG anti-group II responses. The specificitiesof nine anti-Der p II IgE mAbs raised in A/J mice were compared with specificities of seven mAbs produced previously. Most mAbs (11 of 16) recognized common epitopes on Der p II and Der f II: three were specific to Der p II, and two showed high binding to Der f II. Epitope analysis showed that the mAbs defined four cross-reactive, nonoverlapping sites on the group II allergens. Binding of several combinations of mAbs was compared, and a two-site ELISA for group II antigens was developed. Linear regression analysis showed an excellent correlation betweenresults of this assay and group II radioimmunoassay of house dust samples (n = 40, r = 0.85, p < 0.001). Conclusions: There are multiple cross-reactive B-cell epitopes on group II allergens. The group II ELISAhas several important applications including assessment of environmental allergen exposure, monitoring of the efficacy of avoidance procedures, and standardization of commercial mite allergen extracts.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/05/20 alle ore 13:43:06