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Titolo:
THE EBNA-2 ARGININE-GLYCINE DOMAIN IS CRITICAL BUT NOT ESSENTIAL FOR B-LYMPHOCYTE GROWTH TRANSFORMATION - THE REST OF REGION-3 LACKS ESSENTIAL INTERACTIVE DOMAINS
Autore:
TONG X; YALAMANCHILI R; HARADA S; KIEFF E;
Indirizzi:
HARVARD UNIV,DEPT GENET & MICROBIOL,12 S THORN BLDG,75 FRANCIS ST BOSTON MA 02115 HARVARD UNIV,DEPT MICROBIOL & MOLEC GENET BOSTON MA 02115 HARVARD UNIV,DEPT MED BOSTON MA 02115
Titolo Testata:
Journal of virology
fascicolo: 10, volume: 68, anno: 1994,
pagine: 6188 - 6197
SICI:
0022-538X(1994)68:10<6188:TEADIC>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPSTEIN-BARR-VIRUS; LATENT MEMBRANE-PROTEIN; BAMHI C-PROMOTER; NUCLEAR ANTIGEN-2; NUCLEOTIDE-SEQUENCE; GENOME ORGANIZATION; CELL-LINE; DNA; EXPRESSION; RNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
64
Recensione:
Indirizzi per estratti:
Citazione:
X. Tong et al., "THE EBNA-2 ARGININE-GLYCINE DOMAIN IS CRITICAL BUT NOT ESSENTIAL FOR B-LYMPHOCYTE GROWTH TRANSFORMATION - THE REST OF REGION-3 LACKS ESSENTIAL INTERACTIVE DOMAINS", Journal of virology, 68(10), 1994, pp. 6188-6197

Abstract

Since deletion of region 3 (amino acids [aa] 333 to 425) of Epstein-Barr virus nuclear protein 2 (EBNA-2) results in EBV recombinants whichcannot transform primary B lymphocytes (J. I. Cohen, F. Wang, and E. Kieff, J. Virol. 65:2545-2554, 1991), the role of domains of region 3 was investigated. Deletion of the Arg-Gly repeat domain, R-337GQSRGRGRGRGRGRGKG354, results in EBV recombinants that transform primary B lymphocytes with modestly decreased activity. The transformed cells grow slowly and are difficult to expand. EBNA-2 deleted for the Arg-Gly domain does not associate with the nuclear chromatin fraction. The Arg-Gly repeat has an intrinsic ability to bind to histone H1, to other proteins, including EBNA-1, and to nucleic acids, especially poly(G). Two independent deletions of each part of the rest of region 3 (aa 359 to 383 and 385 to 430) have little effect on transformation, while deletion of the rest of region 3 (aa 361 to 425) as a single segment substantially reduces transformation efficiency. EBNA-2 deleted for all of region 3 can still transactivate the LMP1 promoter in transient expression assays but is less active than EBNA-2 in transactivating the BamHI-C promoter. EBNA-2 deleted for the Arg-Gly domain is better than EBNA-2 at transactivating the LMP1 promoter and is as active as EBNA-2 in transactivating the BamHI-C promoter. These data are most compatible with a model in which the Arg-Gly domain of region 3 is a modulator of EBNA-2 interactions and activities, while the rest of region 3 is important in positioning the region 2 J kappa binding domain relative to the region 4 acidic transactivating domain. Despite the null phenotype of the region 3 deletion, region 3 is unlikely to mediate essential interactions with other proteins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 13:18:39