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Titolo:
AUTORADIOGRAPHIC ANALYSIS OF L-TYPE AND N-TYPE VOLTAGE-DEPENDENT CALCIUM-CHANNEL BINDING IN CANINE BRAIN AFTER GLOBAL CEREBRAL ISCHEMIA REPERFUSION/
Autore:
PERRY DC; WEI HF; ROSENTHAL RE; FISKUM G;
Indirizzi:
GEORGE WASHINGTON UNIV,MED CTR,DEPT PHARMACOL,2300 I ST NW WASHINGTONDC 20037 GEORGE WASHINGTON UNIV,MED CTR,DEPT BIOCHEM & MOLEC BIOL WASHINGTON DC 20037 GEORGE WASHINGTON UNIV,MED CTR,DEPT EMERGENCY MED WASHINGTON DC 20037
Titolo Testata:
Brain research
fascicolo: 1-2, volume: 657, anno: 1994,
pagine: 65 - 72
SICI:
0006-8993(1994)657:1-2<65:AAOLAN>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
OMEGA-CONOTOXIN GVIA; RAT-BRAIN; ANTAGONIST; ISCHEMIA; RECEPTORS; VISUALIZATION; NITRENDIPINE; LOCALIZATION; NIMODIPINE; DENDRITES;
Keywords:
GLOBAL CEREBRAL ISCHEMIA; AUTORADIOGRAPHY; VOLTAGE-DEPENDENT CALCIUM CHANNEL; HIPPOCAMPUS; PN200-110 (ISRADIPINE); OMEGA-CONOTOXIN GVIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
D.C. Perry et al., "AUTORADIOGRAPHIC ANALYSIS OF L-TYPE AND N-TYPE VOLTAGE-DEPENDENT CALCIUM-CHANNEL BINDING IN CANINE BRAIN AFTER GLOBAL CEREBRAL ISCHEMIA REPERFUSION/", Brain research, 657(1-2), 1994, pp. 65-72

Abstract

Binding of antagonists to L- and N-type voltage-dependent calcium channels (VDCC) was measured in canine brain following global ischemia and reperfusion. Ischemia was induced by 10 min cardiac arrest, followedby restoration of spontaneous circulation for periods of up to 24 h. Binding of [H-3]PN200-110 and [I-125]omega-conotoxin GVIA to frozen sections from hippocampus, striatum, parietal cortex and temporal cortexwas analyzed using quantitative receptor autoradiography. The bindingpatterns of the two radioligands were similar in cortex and striatum,but differed in hippocampus. In the latter tissue, [I-125]omega-conotoxin GVIA binding was dense over synaptic regions, especially the presynaptic polymorph layer of the dentate gyrus, but was virtually absentover cell body layers. In contrast, [H-3]PN200-110 binding was more homogenously distributed, with highest binding in the molecular layer of the dentate gyrus. The binding of [I-125]omega-conotoxin GVIA was not different from sham controls at any time point following cardiac arrest. [H-3]PN200-110 binding was decreased in each region immediately following ischemia, recovering within 30 min of recirculation. These findings are in contrast to earlier findings of rapid increases in L-type VDCC binding to membrane fractions obtained from cortex and striatumin this model, and suggest that the previously detected increases maybe due to a redistribution of channels from subcellular compartments to the plasma membrane during ischemia.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 19:40:12