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Titolo:
STRUCTURE AND CHROMOSOMAL LOCALIZATION OF CYP6A1, A CYTOCHROME P450-ENCODING GENE FROM THE HOUSE-FLY
Autore:
COHEN MB; KOENER JF; FEYEREISEN R;
Indirizzi:
UNIV ARIZONA,DEPT ENTOMOL,FORBES 410 TUCSON AZ 85721 UNIV ARIZONA,DEPT ENTOMOL TUCSON AZ 85721 UNIV ARIZONA,CTR INSECT SCI TUCSON AZ 85721
Titolo Testata:
Gene
fascicolo: 2, volume: 146, anno: 1994,
pagine: 267 - 272
SICI:
0378-1119(1994)146:2<267:SACLOC>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
INSECTICIDE RESISTANCE; MUSCA-DOMESTICA; EXPRESSION; TRANSCRIPTION; SEQUENCE; PLAYS; CDNA;
Keywords:
MUSCA DOMESTICA; DNA SEQUENCING; INSECTICIDE RESISTANCE; BARBIE BOX; OVEREXPRESSION; TRANS-ACTING FACTOR; PHENOBARBITAL INDUCTION; PCR;
Tipo documento:
Note
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
M.B. Cohen et al., "STRUCTURE AND CHROMOSOMAL LOCALIZATION OF CYP6A1, A CYTOCHROME P450-ENCODING GENE FROM THE HOUSE-FLY", Gene, 146(2), 1994, pp. 267-272

Abstract

We determined the sequence of a cytochrome P450-encoding gene, CYP6A1, in an insecticide-resistant strain (Rutgers) and in insecticide-susceptible strains (aabys and sbo) of the house fly Musca domestica. The deduced amino acid (aa) sequence of CYP6A1 is 98% identical between Rutgers and aabys, and it is identical between Rutgers and sbo. Differences in aa sequence occur in regions that are not thought to participate in the active site. CYP6A1 has a single intron, 60 bp in length. It does not occur in a position homologous to intron sites in genes of the related CYP3 family or other vertebrate P450 families. We mapped thetranscription start point (tsp), TATA box and barbie box [IShaw and Fulco, J. Biol. Chem. 269 (1993) 2997-3004] of this phenobarbital-inducible gene. Although overexpression of CYP6A1 in strain Rutgers is controlled by a locus on chromosome II [Carino et al., Insect Biochem. Mel. Biol. 24 (1994) 411-418], we have localized CYP6A1 to chromosome V, thus showing that a trans-acting factor controls the overexpression ofthe gene in the Rutgers strain.

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Documento generato il 09/07/20 alle ore 01:34:17