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Titolo:
CLONING OF THE WIDE SPECTRUM AMIDASE GENE FROM BREVIBACTERIUM SP R312BY GENETIC COMPLEMENTATION - OVEREXPRESSION IN BREVIBACTERIUM SP AND ESCHERICHIA-COLI
Autore:
AZZA S; BIGEY F; ARNAUD A; GALZY P;
Indirizzi:
ECOLE NATL SUPER AGRON MONTIPELLIER,CHAIR MICROBIOL IND & GENET MICOORGANISMES,PL VIALA F-34060 MONTPELLIER 1 FRANCE ECOLE NATL SUPER AGRON MONTIPELLIER,CHAIR MICROBIOL IND & GENET MICOORGANISMES F-34060 MONTPELLIER 1 FRANCE
Titolo Testata:
FEMS microbiology letters
fascicolo: 1-2, volume: 122, anno: 1994,
pagine: 129 - 136
SICI:
0378-1097(1994)122:1-2<129:COTWSA>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRILE HYDRATASE; ACYLAMIDE AMIDOHYDROLASE; PSEUDOMONAS-AERUGINOSA; PLASMID; SEQUENCE; DNA; PURIFICATION; VECTORS; STRAIN;
Keywords:
BREVIBACTERIUM SP; AMIE GENE CLONING; COMPLEMENTATION AND EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
S. Azza et al., "CLONING OF THE WIDE SPECTRUM AMIDASE GENE FROM BREVIBACTERIUM SP R312BY GENETIC COMPLEMENTATION - OVEREXPRESSION IN BREVIBACTERIUM SP AND ESCHERICHIA-COLI", FEMS microbiology letters, 122(1-2), 1994, pp. 129-136

Abstract

The amiE gene of Brevibacterium sp. R312 encoding wide spectrum amidase was isolated by complementation of a Brevibacterium sp. mutant using a plasmid gene bank of chromosomal DNA. The amiE structural gene andits promoter were localized on a 1.8-kb fragment by subsequent subcloning and complementation studies. Another promoter localized in the pSR 1 fragment of the cloning vector was shown to be able to control amiE gene expression. In Brevibacterium sp., the investigation of amidaseactivities related to one copy of the gene suggested that the regulation of the amiE gene expression was under negative control. High expression levels have been obtained in Brevibacterium sp. and, after substitution of the amiE promoter by the tac promoter, in Escherichia coil.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/05/20 alle ore 12:36:00