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Titolo:
ACTIVATED KI-RAS COMPLEMENTS ERYTHROPOIETIN SIGNALING IN CTLL-2 CELLS, INDUCING TYROSINE PHOSPHORYLATION OF A 160-KDA PROTEIN
Autore:
YAMAMURA Y; NODA M; IKAWA Y;
Indirizzi:
TOKYO MED & DENT UNIV,SCH MED,DEPT BIOCHEM,BUNKYO KU,YUSHIMA 1-5-45 TOKYO 113 JAPAN TOKYO MED & DENT UNIV,SCH MED,DEPT BIOCHEM,BUNKYO KU TOKYO 113 JAPAN INST PHYS & CHEM RES,TSUKUBA LIFE SCI CTR,MOLEC ONCOL LAB TSUKUBA IBARAKI 305 JAPAN JAPANESE FDN CANC RES,INST CANC,DEPT VIRAL ONCOL,TOSHIMA KU TOKYO 170JAPAN
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 19, volume: 91, anno: 1994,
pagine: 8866 - 8870
SICI:
0027-8424(1994)91:19<8866:AKCESI>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERLEUKIN-2 RECEPTOR; ERYTHROID-CELLS; SARCOMA VIRUS; KINASE; GROWTH; GENE; BINDING; FAMILY; PROLIFERATION; GLYCOPROTEIN;
Keywords:
ERYTHROPOIETIN RECEPTOR; JAK2 PROTEIN KINASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
Y. Yamamura et al., "ACTIVATED KI-RAS COMPLEMENTS ERYTHROPOIETIN SIGNALING IN CTLL-2 CELLS, INDUCING TYROSINE PHOSPHORYLATION OF A 160-KDA PROTEIN", Proceedings of the National Academy of Sciences of the United Statesof America, 91(19), 1994, pp. 8866-8870

Abstract

We have previously shown that expression of erythropoietin (EPO) receptor (EPOR) alone failed to confer EPO responsiveness on the interleukin 2-dependent T-cell line CTLL-2, whereas the introduction of the EPOR into interleukin 3 -dependent pro-B-cell lines, such as BAF-B03, allowed the cells to proliferate in response to EPO. Here, we report thatadditional expression of v-Ki-Ras conferred EPO-dependent growth on CTLL-2 cells expressing the EPOR, with additional formation of a high-affinity EPOR. To investigate possible mechanisms of EPOR downstream signaling induced by v-Ki-Ras expression in these CTLL-2-derived cells, we carried out anti-phosphotyrosine inmunoblot analysis of the EPOR complex immunoprecipitated with anti-EPOR antibody from lysates of cellswith and without cytokine stimulation, revealing two 16O-kDa and 130-kDa phosphotyrosyl proteins. An anti-JAK2 antibody did not react with these proteins, suggesting that they may represent cellular componentsinvolved in an EPO-EPOR signaling pathway induced by v-Ki-Ras. Similar phosphotyrosyl proteins were present among Friend erythroleukemia cell lines, in which the Friend virus gp55/EPOR complex on the cell surface constitutively sends signals for cell growth.

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Documento generato il 01/04/20 alle ore 22:08:42