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Titolo:
SYNAPTOTAGMIN-I IS A HIGH-AFFINITY RECEPTOR FOR CLATHRIN-AP-2 - IMPLICATIONS FOR MEMBRANE RECYCLING
Autore:
ZHANG JZ; DAVLETOV BA; SUDHOF TC; ANDERSON RGW;
Indirizzi:
UNIV TEXAS,SW MED CTR,DEPT CELL BIOL & NEUROSCI DALLAS TX 75235 UNIV TEXAS,SW MED CTR,DEPT MOLEC GENET DALLAS TX 75235 UNIV TEXAS,SW MED CTR,HOWARD HUGHES MED INST DALLAS TX 75235
Titolo Testata:
Cell
fascicolo: 5, volume: 78, anno: 1994,
pagine: 751 - 760
SICI:
0092-8674(1994)78:5<751:SIAHRF>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
COATED VESICLES; NEUROTRANSMITTER RELEASE; INVITRO BINDING; GOLGI MEMBRANES; PROTEIN; EXOCYTOSIS; DOMAIN; FUSION; PITS; DROSOPHILA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
J.Z. Zhang et al., "SYNAPTOTAGMIN-I IS A HIGH-AFFINITY RECEPTOR FOR CLATHRIN-AP-2 - IMPLICATIONS FOR MEMBRANE RECYCLING", Cell, 78(5), 1994, pp. 751-760

Abstract

In nerve terminals, Ca2+-stimulated synaptic vesicle exocytosis is rapidly followed by endocytosis. Synaptic vesicle endocytosis requires clathrin-coated pits similar to receptor-mediated endocytosis in fibroblasts. Binding of clathrin AP-2 (adaptor complex) to an unidentified high affinity membrane receptor appears to be necessary for coated pit assembly in fibroblasts. We now show that synaptic vesicles have a high affinity AP-2 site (K-D, similar to 1 x 10(-10) M) similar to the one observed in fibroblasts. Using a combination of competition and direct binding assays, we demonstrate that synaptotagmin I, an intrinsic membrane protein of synaptic vesicles, has all of the properties of theAP-2 receptor and that AP-2 binds to the second C-2 domain in the molecule. Thus, synaptotagmin I may be a multifunctional protein with a function in endocytosis in addition to the previously proposed role in exocytosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 13:00:46