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Titolo:
CHARACTERIZATION OF THE FUNCTIONAL-ROLE OF E-BOX ELEMENTS FOR THE TRANSCRIPTIONAL ACTIVITY OF RAT ACETYLCHOLINE-RECEPTOR EPSILON-SUBUNIT AND GAMMA-SUBUNIT GENE PROMOTERS IN PRIMARY MUSCLE-CELL CULTURES
Autore:
DURR I; NUMBERGER M; BERBERICH C; WITZEMANN V;
Indirizzi:
MAX PLANCK INST MED RES,ZELLPHYSIOL ABT,POSTFACH 103820 D-69028 HEIDELBERG GERMANY MAX PLANCK INST MED RES,ZELLPHYSIOL ABT D-69028 HEIDELBERG GERMANY
Titolo Testata:
European journal of biochemistry
fascicolo: 2, volume: 224, anno: 1994,
pagine: 353 - 364
SICI:
0014-2956(1994)224:2<353:COTFOE>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
PREFERENTIAL SYNAPTIC EXPRESSION; MESSENGER-RNA LEVELS; ALPHA-SUBUNIT; DELTA-SUBUNIT; BETA-SUBUNIT; ELECTRICAL-ACTIVITY; MOUSE MUSCLE; DEVELOPMENTAL REGULATION; DIFFERENTIATION PROGRAM; NEUROMUSCULAR SYNAPSES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
67
Recensione:
Indirizzi per estratti:
Citazione:
I. Durr et al., "CHARACTERIZATION OF THE FUNCTIONAL-ROLE OF E-BOX ELEMENTS FOR THE TRANSCRIPTIONAL ACTIVITY OF RAT ACETYLCHOLINE-RECEPTOR EPSILON-SUBUNIT AND GAMMA-SUBUNIT GENE PROMOTERS IN PRIMARY MUSCLE-CELL CULTURES", European journal of biochemistry, 224(2), 1994, pp. 353-364

Abstract

The expression of gamma and epsilon subunits of the acetylcholine receptor from mammalian skeletal muscle is regulated independently duringmyogenic differentiation and innervation. Genomic DNA fragments containing 5'-flanking sequences of the epsilon-subunit and gamma-subunit genes were characterised by a series of 5' deletions fused to the chloramphenicol-acetyltransferase gene and transiently expressed by transfection of primary cultures of rat muscle cells and non-muscle cells. A 6.3-kb epsilon-subunit fragment can be reduced to yield a 270-bp fragment that confers 5-10-times higher expression levels in muscle cells compared to in non-muscle cells. The region composed of nucleotides -185 to -128 increases the transcriptional activity moderately while the 14-bp palindrome containing a single E box at nucleotides -88 to -83 may interact with the promoter but has no enhancer properties in musclecells. From a 1.1-kb genomic fragment of the gamma-subunit gene, 167 bp were sufficient for muscle-specific expression. Two promoter-proximal E-box elements enhance promoter activity in muscle and mediate transactivation by myogenic factors. Myogenin and myf5 were much more efficient than MRF4 or MyoD1 which exerted only little transactivation. Cotransfection experiments show that increased expression of Id in primary muscle cells inhibits chloramphenicol-acetyltransferase expression mediated by the gamma-subunit gene promoter and support the view that myogenic factors play an important role in the transcriptional regulation of the gamma-subunit gene.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 14:27:04