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Titolo:
MITOCHONDRIAL MODIFICATIONS DURING RAT THYMOCYTE APOPTOSIS - A STUDY AT THE SINGLE-CELL LEVEL
Autore:
COSSARIZZA A; KALASHNIKOVA G; GRASSILLI E; CHIAPPELLI F; SALVIOLI S; CAPRI M; BARBIERI D; TROIANO L; MONTI D; FRANCESCHI C;
Indirizzi:
UNIV MODENA,DEPT BIOMED SCI,GEN PATHOL SECT,VIA CAMPI 287 I-41100 MODENA ITALY RUSSIAN ACAD MED SCI,CANC RES CTR MOSCOW 115552 RUSSIA UNIV CALIF LOS ANGELES,DEPT ANAT & CELL BIOL LOS ANGELES CA 90024
Titolo Testata:
Experimental cell research
fascicolo: 1, volume: 214, anno: 1994,
pagine: 323 - 330
SICI:
0014-4827(1994)214:1<323:MMDRTA>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
J-AGGREGATE FORMATION; FLOW-CYTOMETRY; LIPID-PEROXIDATION; FLUORESCENT-PROBE; PARINARIC ACID; DEATH; SURFACE; BCL-2; ACTIVATION; ELEGANS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
A. Cossarizza et al., "MITOCHONDRIAL MODIFICATIONS DURING RAT THYMOCYTE APOPTOSIS - A STUDY AT THE SINGLE-CELL LEVEL", Experimental cell research, 214(1), 1994, pp. 323-330

Abstract

Apoptosis is an active type of cell death, occurring under several physiological and pathological conditions, The role of cellular organelles such as mitochondria in this process is still an open question. We recently described a new method to measure mitochondrial membrane potential in intact cells using dow cytometry. Using this method we studied alterations of mitochondrial membrane potential in a classical modelof apoptosis, i.e., dexamethasone-treated rat thymocytes. Moreover, apoptosis induced by heat shock was also studied in the same cells. Mitochondria are functionally intact during the early phases of apoptosis, when DNA fragmentation occurs, whereas early alteration in their potential and mass takes place after DNA damage. According to flow-cytometric analysis, the presence of a hypodiploid peak, an index of nuclearDNA loss, predates depolarization of mitochondrial membrane and decrease of mitochondrial mass. Loss of plasma membrane integrity, which indicates cell death and is revealed by the permeability to propidium iodide, eventually follows. All these phenomena are more evident in dexamethasone-treated cells than in heat-shocked cells, Thus, in these types of apoptosis the involvement of mitochondria is apparently not a primary event. (C) 1994 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 07:34:06