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Titolo:
CCAAT BOX-BINDING PROTEIN NF-Y FACILITATES IN-VIVO RECRUITMENT OF UPSTREAM DNA-BINDING TRANSCRIPTION FACTORS
Autore:
WRIGHT KL; VILEN BJ; ITOHLINDSTROM Y; MOORE TL; LI GX; CRISCITIELLO M; COGSWELL P; CLARKE JB; TING JPY;
Indirizzi:
UNIV N CAROLINA,LINEBERGER COMPREHENS CANC CTR,DEPT MICROBIOL IMMUNOL,CB 7295 CHAPEL HILL NC 27599 UNIV N CAROLINA,LINEBERGER COMPREHENS CANC CTR,DEPT MICROBIOL IMMUNOLCHAPEL HILL NC 27599
Titolo Testata:
EMBO journal
fascicolo: 17, volume: 13, anno: 1994,
pagine: 4042 - 4053
SICI:
0261-4189(1994)13:17<4042:CBPNFI>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
CLASS-II GENES; MHC CLASS-II; TUMOR VIRUS PROMOTER; DR-ALPHA GENE; INTERFERON-GAMMA; MAMMALIAN-CELLS; POSITIONED NUCLEOSOMES; SYNERGISTIC ACTIVATION; HETEROLOGOUS SUBUNITS; ALBUMIN PROMOTER;
Keywords:
CCAAT; HLA-DTC; IN VIVO FOOTPRINT; NF-Y; TRANSCRIPTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
79
Recensione:
Indirizzi per estratti:
Citazione:
K.L. Wright et al., "CCAAT BOX-BINDING PROTEIN NF-Y FACILITATES IN-VIVO RECRUITMENT OF UPSTREAM DNA-BINDING TRANSCRIPTION FACTORS", EMBO journal, 13(17), 1994, pp. 4042-4053

Abstract

NF-Y binds a CCAAT motif found in many eukaryotic polymerase II-dependent promoters, In the HLA-DRA promoter it has been demonstrated that stereo-specific alignment between this motif and the upstream elementsX1 and X2 is required for activation. To study the underlying mechanism for this requirement, a panel of transfected cell lines that maintained integrated, wild-type and mutant promoters were analyzed by in vivo genomic footprinting. Cell lines harboring a mutated CCAAT element exhibited a loss of interactions at the CCAAT site, as expected, and no transcriptional activity. Most importantly, mutation of the CCAAT sequence nearly abolished in vivo binding at the X1 and X2 sites, while mutations of X1 and X2 had little effect on CCAAT box binding. However, X1 and X2 binding was interdependent. In vivo, X1 binding activitiesare known to be stabilized by NF-Y binding. Interaction between NF-Y and X box binding proteins was demonstrated by reciprocal co-immunoprecipitation in the absence of DNA and co-affinity purification in the presence of DNA. Collectively, these studies indicate that occupancy ofthe CCAAT element represents an early event affecting other protein-DNA interactions and suggest that NF-Y stabilizes and interacts with X box factors to mediate this function. These findings may represent a common theme among promoters containing a CCAAT element.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 07:47:39