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Titolo:
ALTERATION OF INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR AFTER 6-HOUR HEMISPHERIC ISCHEMIA IN THE GERBIL BRAIN
Autore:
NAGATA E; TANAKA K; GOMI S; MIHARA B; SHIRAI T; NOGAWA S; NOZAKI H; MIKOSHIBA K; FUKUUCHI Y;
Indirizzi:
KEIO UNIV,SCH MED,DEPT NEUROL,SHINJUKU KU,35 SHINANOMACHI TOKYO 160 JAPAN MIHARA MEM HOSP ISESAKI GUNMA JAPAN UNIV TOKYO,INST MED SCI,DEPT MOLEC NEUROBIOL TOKYO JAPAN
Titolo Testata:
Neuroscience
fascicolo: 4, volume: 61, anno: 1994,
pagine: 983 - 990
SICI:
0306-4522(1994)61:4<983:AOI1RA>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
FREE FATTY-ACIDS; DELAYED NEURONAL DEATH; CEREBRAL BLOOD-FLOW; RAT HIPPOCAMPUS; AUTORADIOGRAPHIC ANALYSIS; 2ND-MESSENGER SYSTEMS; INSP3 RECEPTOR; P400 PROTEIN; MOUSE-BRAIN; CALCIUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
E. Nagata et al., "ALTERATION OF INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR AFTER 6-HOUR HEMISPHERIC ISCHEMIA IN THE GERBIL BRAIN", Neuroscience, 61(4), 1994, pp. 983-990

Abstract

In order to evaluate the influence of cerebral ischemia on the inositol 1,4,5-trisphosphate receptor, the alterations of inositol I,4,5-trisphosphate receptor binding sites and local cerebral blood flow were examined 6 h after occlusion of the right common carotid artery in the gerbil brain. The autoradiographic method developed in our laboratory enabled us to measure both parameters within the same brain. Animals attaining ischemic scores of more than 5, as assessed 1 h after occlusion, were utilized. The local cerebral blood flow was measured 6 h after occlusion by the C-14]iodoantipyrine method. The inositol 1,4,5-trisphosphate binding sites were evaluated in vitro using [H-3]inositol 1,4,5-trisphosphate as a specific ligand. The local cerebral blood flow fell below 15 ml/100 g per min in most of the cerebral regions on the occluded side. In contrast, a significant reduction in inositol l,4,5-trisphosphate binding sites was noted only in the hippocampus CA 1 on the occluded side. Inositol 1,4,5-trisphosphate binding tended to decrease when the values of local cerebral blood flow were below 20 ml/100g per min in this region. On the other hand, the inositol 1,4,5-trisphosphate receptor immunoreactivity in the brain examined with a monoclonal antibody against inositol 1,4,5-trisphosphate receptor protein did not reveal any differences between the ischemia and sham groups on both sides, suggesting that the inositol 1,4,5-trisphosphate receptors may not undergo significant morphological degradation. These findings indicate that the suppression of inositol 1,4,5-trisphosphate binding in the hippocampus CA 1 may be attributable to a regionally specific perturbation of the inositol 1,4,5-trisphosphate metabolism in this region. Such perturbation may be closely associated with the pathophysiological mechanism of selective ischemic vulnerability of this region.

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Documento generato il 11/07/20 alle ore 11:16:27