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Titolo:
ANALYSIS OF COMPUTER-PREDICTED ANTIBODY INDUCING EPITOPE ON JAPANESE ENCEPHALITIS-VIRUS
Autore:
KUTUBUDDIN M; GORE MM; BANERJEE K; GHOSH SN; KOLASKAR AS;
Indirizzi:
NATL INST VIROL,20-A DR AMBEDKAR ROAD POONA 411001 INDIA UNIV POONA,DEPT ZOOL,BIOTECHNOL TRAINING PROGRAMME POONA 411007 MAHARASHTRA INDIA
Titolo Testata:
Acta virologica
fascicolo: 6, volume: 37, anno: 1993,
pagine: 417 - 428
SICI:
0001-723X(1993)37:6<417:AOCAIE>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENVELOPE PROTEIN; MONOCLONAL-ANTIBODIES; E-GLYCOPROTEIN; STRAIN; FLAVIVIRUS; SEQUENCES;
Keywords:
JAPANESE ENCEPHALITIS VIRUS; SYNTHETIC PEPTIDE; B-CELL EPITOPE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
22
Recensione:
Indirizzi per estratti:
Citazione:
M. Kutubuddin et al., "ANALYSIS OF COMPUTER-PREDICTED ANTIBODY INDUCING EPITOPE ON JAPANESE ENCEPHALITIS-VIRUS", Acta virologica, 37(6), 1993, pp. 417-428

Abstract

Theoretical methods to delineate antibody inducing epitopes have beenemployed to predict antigenic determinants on envelope glycoprotein (gpE) of Japanese encephalitis (JE), West Nile (WN) and Dengue (DEN) I-IV viruses. A predicted region on JE virus gpE 74CPTTGEAHNEKRAD87 was synthesized, conjugated to KLH (KLH-peptide) and used in immunization of mice. A mouse monoclonal antibody (MoAb IVB4) reactive to the peptide was also found to react with native JE virus gpE. Characterization of the idiotypic (ID) determinants with the help of polyclonal domain-specific anti-ID antibodies revealed that polyclonal anti-KLH-peptide antibodies and MoAb IVB4 are flavivirus-cross-reactive to Hx and NHx domains, respectively. The region 74-87 in JE virus gpE has been mappedas a linking area between Hx and NHx domains. Reactivity of the peptide with sera from JE patients and vaccinees also indicated the feasibility of using predicted peptides for diagnostic and prophylastic purposes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 04:54:51