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Titolo:
OVER-EXPRESSION OF CUG-INITIATED OR AUG-INITIATED FORMS OF BASIC FIBROBLAST GROWTH-FACTOR IN CARDIAC MYOCYTES RESULTS IN SIMILAR EFFECTS ONMITOSIS AND PROTEIN-SYNTHESIS BUT DISTINCT NUCLEAR MORPHOLOGIES
Autore:
PASUMARTHI KBS; DOBLE BW; KARDAMI E; CATTINI PA;
Indirizzi:
UNIV MANITOBA,DEPT PHYSIOL,770 BANNATYNE AVE WINNIPEG R3E 0W3 MB CANADA UNIV MANITOBA,DEPT PHYSIOL WINNIPEG R3E 0W3 MB CANADA ST BONIFACE GEN HOSP,RES CTR,DIV CARDIOVASC SCI WINNIPEG R2H 2A6 MB CANADA
Titolo Testata:
Journal of Molecular and Cellular Cardiology
fascicolo: 8, volume: 26, anno: 1994,
pagine: 1045 - 1060
SICI:
0022-2828(1994)26:8<1045:OOCOAF>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA; CELLS; TRANSLATION; FGF; TRANSLOCATION; PROLIFERATION; COMPLEMENTARY; LOCALIZATION; INHIBITION; VENTRICLES;
Keywords:
CARDIAC MYOCYTES; BASIC FIBROBLAST GROWTH FACTOR; GENE TRANSFER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
K.B.S. Pasumarthi et al., "OVER-EXPRESSION OF CUG-INITIATED OR AUG-INITIATED FORMS OF BASIC FIBROBLAST GROWTH-FACTOR IN CARDIAC MYOCYTES RESULTS IN SIMILAR EFFECTS ONMITOSIS AND PROTEIN-SYNTHESIS BUT DISTINCT NUCLEAR MORPHOLOGIES", Journal of Molecular and Cellular Cardiology, 26(8), 1994, pp. 1045-1060

Abstract

Initiation of translation from alternate codons in the same mRNA results in multiple forms of basic fibroblast growth factor (bFGF). High molecular weight species of bFGF make use of leucine translation initiation sites located upstream of the methionine residue used to produce the 18 kiloDalton (kDa) form. Although the addition of exogenous 18 kDa bFGF is known to stimulate DNA synthesis and proliferation of several cell types including embryonic chicken cardiac myocytes, little is known about the role of high molecular weight forms of bFGF. We modified the rat bFGF cDNA to yield high (22/21.5 kDa) or low (18 kDa) molecular weight species of bFGF. Expression of 22/21.5 kDa or 18 kDa bFGF in transfected embryonic chicken ventricular myocyte cultures was confirmed by protein blotting. Expression of both high and low molecular weight species of bFGF was associated with (i) a three-fold increase in overall thymidine incorporation as well as cardiomyocyte labelling index (fraction of cardiomyocyte nuclei incorporating tritiated thymidine); (ii) a two- to three-fold increase in cell number; (iii) an eight-fold increase in protein synthesis; and (iv) a three-fold decrease in myosin accumulation. Subcellular Localization of bFGF in the transfected myocyte cultures was also assessed by immunofluorescence microscopy. Over-expression of cDNAs yielding high molecular weight bFGF resultedin predominantly nuclear bFGF staining. By contrast, both cytoplasmicand nuclear staining were observed following oner-expression of 18 kDa bFGF. Over-expression of 22/21.5 kDa bFGF was associated with the formation of multiple DNA-containing ''clumps'' resembling condensed chromatin in cardiac myocyte nuclei. These DNA ''clumps'' were not observed in cardiac myocyte cultures overexpressing 18 kDa bFGF. These data indicate that over-expression of high as well as low molecular weight forms of bFGF can stimulate cardiac myocyte proliferative potential and decrease myosin accumulation. However, these forms possess distinct subcellular localizations and can have different biological functions in the nucleus.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 04:31:06