Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
MULTICENTER EVALUATION OF QUANTIFICATION METHODS FOR PLASMA HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 RNA
Autore:
LIN HJ; MYERS LE; YENLIEBERMAN B; HOLLINGER FB; HENRARD D; HOOPER CJ; KOKKA R; KWOK S; RASHEED S; VAHEY M; WINTERS MA; MCQUAY LJ; NARA PL; REICHELDERFER P; COOMBS RW; JACKSON JB;
Indirizzi:
CASE WESTERN RESERVE UNIV,INST PATHOL,2085 ADELBERT RD CLEVELAND OH 44106 CASE WESTERN RESERVE UNIV,INST PATHOL CLEVELAND OH 44106 BAYLOR COLL MED,DIV MOLEC VIROL HOUSTON TX 77030 RES TRIANGLE INST RES TRIANGLE PK NC 27709 UNIV CLEVELAND HOSP,CLEVELAND CLIN FDN,DEPT PATHOL CLEVELAND OH 44106 ABBOTT LABS ABBOTT PK IL 60064 UNIV WASHINGTON,RETROVIRUS LAB SEATTLE WA 98195 CHIRON CORP EMERYVILLE CA 94608 ROCHE MOLEC SYST ALAMEDA CA 00000 UNIV SO CALIF,SCH MED,VIRAL ONCOL & AIDS RES LAB LOS ANGELES CA 00000 STANFORD UNIV,SCH MED,CTR AIDS RES PALO ALTO CA 94304 WALTER REED ARMY INST RES,DIV RETROVIROL ROCKVILLE MD 00000 NIAID,DIV AIDS ROCKVILLE MD 00000 NCI,TUMOR CELL BIOL LAB,VIRUS BIOL SECT FREDERICK MD 21701
Titolo Testata:
The Journal of infectious diseases
fascicolo: 3, volume: 170, anno: 1994,
pagine: 553 - 562
SICI:
0022-1899(1994)170:3<553:MEOQMF>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; HIV-INFECTED INDIVIDUALS; CLINICAL-TRIALS GROUP; REVERSE-TRANSCRIPTASE; COMBINATION THERAPY; VIREMIA; ZIDOVUDINE; ANTIGEN; VIRIONS; ASSAY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
H.J. Lin et al., "MULTICENTER EVALUATION OF QUANTIFICATION METHODS FOR PLASMA HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 RNA", The Journal of infectious diseases, 170(3), 1994, pp. 553-562

Abstract

Six procedures for quantifying plasma human immunodeficiency virus type 1 (HIV-1) RNA were evaluated by nine laboratories. The procedures differed in their sample volume and preparation of samples and methods of amplification and detection. Coded samples in a 10-fold dilution series of HIV-1-spiked plasma were correctly ranked by all six procedures. Subsequently, coded duplicate plasma samples from 16 HIV-1-infectedpatients were tested using a common set of standards. Several HIV-1 RNA procedures were sufficiently reproducible so that an empiric 4-foldchange could be viewed as significant. HIV-1 RNA levels in the patients (up to 370,000 RNA copies/mL) correlated with proviral HIV-1 DNA and were inversely correlated with CD4 cell counts; HIV-1 RNA assays were more sensitive than plasma viremia, standard p24 antigen, or immune complex-dissociated p24 antigen assays. This study demonstrated that several HIV-1 RNA quantitative assays are ready for use in clinical trials.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 23:37:39