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Titolo:
DIFFERENTIAL REGULATION OF CATION-TRANSPORT OF VASCULAR SMOOTH-MUSCLECELLS IN A HIGH GLUCOSE-CONCENTRATION MILIEU
Autore:
KURIYAMA S; TOKUDOME G; TOMONARI H; UTSUNOMIYA Y; MATSUI K; HASHIMOTO T; SAKAI O;
Indirizzi:
SAISEIKAI CENT HOSP,DIV NEPHROL,MINATO KU,1-4-17 MITA TOKYO 108 JAPAN JIKEI UNIV TOKYO JAPAN
Titolo Testata:
Diabetes research and clinical practice
fascicolo: 2, volume: 24, anno: 1994,
pagine: 77 - 84
SICI:
0168-8227(1994)24:2<77:DROCOV>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
NA+-K+-ATPASE; SKIN FIBROBLASTS; RAT; INVITRO; ABNORMALITIES; INSULIN; INVIVO;
Keywords:
NA-K PUMP; NA-K COTRANSPORT; NA-H ANTIPORT; CA FLUX; VASCULAR SMOOTH MUSCLE CELLS; HIGH GLUCOSE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
16
Recensione:
Indirizzi per estratti:
Citazione:
S. Kuriyama et al., "DIFFERENTIAL REGULATION OF CATION-TRANSPORT OF VASCULAR SMOOTH-MUSCLECELLS IN A HIGH GLUCOSE-CONCENTRATION MILIEU", Diabetes research and clinical practice, 24(2), 1994, pp. 77-84

Abstract

To gain new insights into the pathogenesis of diabetic angiopathy, the influence of high glucose concentration on cation transport of vascular smooth muscle cell (VSMC) membrane was investigated by measuring Na, K and Ca transport in serially passaged cultured VSMC. (1) Na-K pump activity, described as ouabain sensitive Rb-86 uptake, and Na-K cotransport, described as bumetanide sensitive Rb-86 washout of VSMC, grown in high glucose concentration medium (460 mg/dl), was lower than that grown in normal glucose concentration medium (100 mg/dl). A smaller 5-N,N-hexamethylene amiloride (HMA) sensitive Na-22 uptake (Na-H antiport) in high glucose concentration medium accounted for this difference. (2) Ca-45 uptake was also smaller in VSMC cultured in high glucose concentration medium. However, the washout rate constant for Ca-45 wascomparable between high and normal glucose cultured VSMC. (3) Both intracellular concentration of Na and cytosolic free Ca concentration ([Ca](i)) of high glucose cultured VSMC were greater than normal glucosecultured VSMC. (4) Intracellular water volume based on the equilibrium distribution of 3-O-methyl[C-14]glucose was not different between normal and high glucose cultured VSMC. It is concluded that VSMC grown in high glucose concentration milieu manifests a decreased Na-K, and Catransport in conjunction with an increase in intracellular concentration of Na and [Ca](i). These results suggest that high glucose, per se, may alter membrane permeability to cations, possibly leading to changes in VSMC contractility and/or proliferation. This abnormality seen in the diabetic state may closely link to the pathogenesis of diabeticangiopathy, thus as a result risking hypertenion and vascular disease.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 03:53:45