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Titolo:
COMPARISON BETWEEN EXPERIMENTAL AND THEORETICAL PROFILES OF HIGH-CONCENTRATION ELUTION BANDS AND LARGE SYSTEM PEAKS IN NONLINEAR CHROMATOGRAPHY
Autore:
FORNSTEDT T; GUIOCHON G;
Indirizzi:
UNIV TENNESSEE,DEPT CHEM KNOXVILLE TN 37996 UNIV TENNESSEE,DEPT CHEM KNOXVILLE TN 37996 OAK RIDGE NATL LAB,DIV ANALYT CHEM OAK RIDGE TN 37831
Titolo Testata:
Analytical chemistry
fascicolo: 17, volume: 66, anno: 1994,
pagine: 2686 - 2693
SICI:
0003-2700(1994)66:17<2686:CBEATP>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
PAIR ADSORPTION CHROMATOGRAPHY; PERFORMANCE LIQUID-CHROMATOGRAPHY; QUATERNARY AMMONIUM ION; BINARY ELUENT; MOBILE PHASE; COMPRESSION; RETENTION; OPTIMIZATION; REMOXIPRIDE; BIOANALYSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
T. Fornstedt e G. Guiochon, "COMPARISON BETWEEN EXPERIMENTAL AND THEORETICAL PROFILES OF HIGH-CONCENTRATION ELUTION BANDS AND LARGE SYSTEM PEAKS IN NONLINEAR CHROMATOGRAPHY", Analytical chemistry, 66(17), 1994, pp. 2686-2693

Abstract

A detailed experimental investigation of the profiles of system peaksand single solute bands at high concentrations was conducted under experimental conditions leading to severe interference between the primary additive system peak and the additive system peak associated with the solute band. These conditions trigger the formation of extremely unusual band shapes. Both additive and solute individual profiles have been recorded separately, using a diode-array detector operating at different wavelengths selected for the selectivity of the response to thetwo different compounds. The profiles recorded are in very good agreement with the profiles calculated from the isotherms of the two compounds, using the equilibrium-dispersive model of chromatography. This result confirms that major distortions of band profiles occur whenever the additive is more strongly adsorbed than the solute by the stationary phase, but its concentration in the mobile phase is high enough for the additive primary system peak to be eluted before the solute peak, with a separation factor close to unity. Although the experiments werecarried out using a reversed-phase ion-pair chromatographic system, the same phenomenon has been shown to occur in normal-phase adsorption chromatography as well. Important band deformation, corresponding to the passage from a Langmuirian to an anti-Langmuirian band profile whenthe sample size is increased may easily take place when these modes of chromatography are used for preparative applications, explaining a certain disfavor among users. Guidelines are suggested to avoid or control these phenomena whose consequences may not be always as disastrousas felt at first glance.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 17:48:50