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Titolo:
LUMINARIN-9 - A NEW FLUORESCENT REAGENT FOR THE PRECOLUMN DERIVATIZATION OF MAIN VOLATILE AND NONVOLATILE N-NITROSAMINES IN RP-HPLC
Autore:
ZHOU ZY; DAUPHIN C; PROGNON P; HAMON M;
Indirizzi:
FAC PHARM CHATENAY MALABRY,CHIM ANALYT LAB 2,5 RUE JB CLEMENT F-92290CHATENAY MALABRY FRANCE FAC PHARM CHATENAY MALABRY,CHIM ANALYT LAB 2 F-92290 CHATENAY MALABRYFRANCE
Titolo Testata:
Chromatographia
fascicolo: 3-4, volume: 39, anno: 1994,
pagine: 185 - 191
SICI:
0009-5893(1994)39:3-4<185:L-ANFR>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIQUID-CHROMATOGRAPHY; NITROSO COMPOUNDS; ACIDS;
Keywords:
COLUMN LIQUID CHROMATOGRAPHY; N-NITROSAMINES; FLUORESCENT DERIVATIVES; LUMINARIN 9; PRECOLUMN DERIVATIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
Z.Y. Zhou et al., "LUMINARIN-9 - A NEW FLUORESCENT REAGENT FOR THE PRECOLUMN DERIVATIZATION OF MAIN VOLATILE AND NONVOLATILE N-NITROSAMINES IN RP-HPLC", Chromatographia, 39(3-4), 1994, pp. 185-191

Abstract

A sensitive precolumn fluorescence derivatization method for low level detection of the volatile (N-nitrosodimethylamine and N-nitrosopyrrolidine) and non-volatile N-nitrosamines (N-nitrosoproline and N-nitrosodiethanolamine) an high-performance liquid chromatography was developed. This method is based on the denitrosation of the compounds of interest by a mixture of hydrobromic acid and acetic acid to produce the corresponding secondary amines. These are, then, able to react with a quinolizinocoumarin derivative (luminarin 9(R)) to form highly fluorescent labelled N-nitrosamine derivatives. The structural elucidation of the luminarin 9(R) derivatives of N-nitrosoproline and N-nitrosodimethylamine by way of example, were established by liquid chromatography mass spectrometry (LC-MS) and by direct chemical ionization - mass spectrometry (CI-MS). The separation, derivatization and detection conditions were optimized for all the studied compounds. The detection limits(signal to noise ratio = 3) were between 0.4 and 1.0 pmol injected depending on the compound. The calibration graphs were linear for derivatized amounts in the range of 0.5-40 nmol for N-nitrosodimethylamine and N-nitrosopyrrolidine, 0.4-20 nmol for N-nitrosoproline and 1.0-40 nmol for N-nitrosodiethanolamine. The repeatability (RSD less than 3.5 %, n = 6) and reproducibility (RSD less than 4.8 %, n = 6) were satisfactory.

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Documento generato il 03/04/20 alle ore 08:00:12