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Titolo:
BROMELAIN PROTEINASES MODULATE THE CD44 EXPRESSION ON HUMAN MOLT-4 8 LEUKEMIA AND SK-MEL-28 MELANOMA-CELLS IN-VITRO/
Autore:
HARRACH T; GEBAUER F; ECKERT K; KUNZE R; MAURER HR;
Indirizzi:
FREE UNIV BERLIN,INST PHARM,PHARMAZEUT BIOCHEM ABT,KELCHSTR 31 D-12169 BERLIN GERMANY FREE UNIV BERLIN,INST PHARM,PHARMAZEUT BIOCHEM ABT D-12169 BERLIN GERMANY IMTOX GMBH D-13355 BERLIN GERMANY
Titolo Testata:
International journal of oncology
fascicolo: 3, volume: 5, anno: 1994,
pagine: 485 - 488
SICI:
1019-6439(1994)5:3<485:BPMTCE>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
CONFER METASTATIC BEHAVIOR; SPLICE VARIANTS;
Keywords:
BROMELAIN; CD44; PROTEINASES; FLOW CYTOMETRY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
16
Recensione:
Indirizzi per estratti:
Citazione:
T. Harrach et al., "BROMELAIN PROTEINASES MODULATE THE CD44 EXPRESSION ON HUMAN MOLT-4 8 LEUKEMIA AND SK-MEL-28 MELANOMA-CELLS IN-VITRO/", International journal of oncology, 5(3), 1994, pp. 485-488

Abstract

CD44 cell surface proteins are involved in leukocyte binding to endothelium and the metastatic spread of tumor cells. Using flow cytometricanalysis (FCMA), we investigated the effects of the proteases bromelain, papain, trypsin, and chymotrypsin on the density of CD44 moleculespresent on human leukemia Molt 4/8 cells. Bromelain was found to be most active in reducing CD44 receptor density. In addition, the effectsof the purified bromelain proteinases F4 and F9 were investigated. OnMolt 4/8 cells crude bromelain and F9, with the highest proteolyticalactivity, were found to be most active in reducing CD44 receptor density with a half maximal value of 1.9 mu g/ml and 2.3 mu g/ml, respectively. On human SK-Mel 28 melanoma cells especially F9 showed a strong effect, with a half maximal value of 1.5 mu g/ml. The implications of the findings are discussed with view of the reported antimetastatic activity of orally administrated bromelain with respect to CD44.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 08:55:32