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Titolo:
GENE-TRANSFER AND THE EXPRESSION OF A FOREIGN GENE IN-VIVO IN POSTMITOTIC NEURONS OF THE ADULT-RAT BRAIN USING THE HEMAGGLUTINATING VIRUS OF THE JAPAN-LIPOSOME METHOD
Autore:
KATO K; YONEDA Y; OKADA Y; KIYAMA H; SHIOSAKA S;
Indirizzi:
NARA INST SCI & TECHNOL,8916-5 TAKAYAMA NARA 63001 JAPAN OSAKA UNIV,SCH MED SUITA OSAKA 565 JAPAN SENRI LIFE SCI FDN TOYONAKA OSAKA 565 JAPAN
Titolo Testata:
Molecular brain research
fascicolo: 3-4, volume: 25, anno: 1994,
pagine: 359 - 363
SICI:
0169-328X(1994)25:3-4<359:GATEOA>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; II MUTANT MICE; CEREBRAL-CORTEX; MOUSE MUSCLE; INVIVO; HEPATITIS; VECTORS; THERAPY; LIVER; PHYSIOLOGY;
Keywords:
LIPOSOME; HEMAGGLUTINATING VIRUS OF JAPAN; GENE TRANSFER; BRAIN; CENTRAL NERVOUS SYSTEM; POSTMITOTIC NEURONS;
Tipo documento:
Note
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
K. Kato et al., "GENE-TRANSFER AND THE EXPRESSION OF A FOREIGN GENE IN-VIVO IN POSTMITOTIC NEURONS OF THE ADULT-RAT BRAIN USING THE HEMAGGLUTINATING VIRUS OF THE JAPAN-LIPOSOME METHOD", Molecular brain research, 25(3-4), 1994, pp. 359-363

Abstract

Neurons in the adult rat brain were transfected in vivo with a simpleplasmid that harbored the gene for beta-galactosidase from Escherichia coli under control of a chicken beta-actin promoter by use of the hemagglutinating virus of Japan (HVJ) and liposomes. Cells that expressed beta-galactosidase were detected only in the target area of the central nervous system for 10 days by light microscopic analysis. Since electron microscopic analysis revealed that the products of the histochemical reaction were predominantly associated with the nuclear membraneand the endoplasmic reticulum of positive cells, it appeared that theproducts were translated endogenously and had not been entrapped by endocytosis. Furthermore, the products were observed in typical neuronal cells with a large, round, and pale nuclei, and with direct axe-somatic and axo-dendritic synaptic contacts. This report suggests the possibility of introducing functionally significant genes into neurons in targeted areas of the adult central nervous system.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 19:53:36