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Titolo:
DIFFERENTIAL EXPRESSION OF GABA(A) RECEPTOR SUBUNIT MESSENGER-RNAS INETHANOL-NAIVE WITHDRAWAL SEIZURE RESISTANT (WSR) VS WITHDRAWAL SEIZURE PRONE (WSP) MOUSE-BRAIN
Autore:
KEIR WJ; MORROW AL;
Indirizzi:
UNIV N CAROLINA,SCH MED,DEPT PSYCHIAT,CB 7175,MED RES BLDG A CHAPEL HILL NC 27599 UNIV N CAROLINA,SCH MED,DEPT PSYCHIAT CHAPEL HILL NC 27599 UNIV N CAROLINA,SCH MED,CTR ALCOHOL STUDIES CHAPEL HILL NC 27599
Titolo Testata:
Molecular brain research
fascicolo: 3-4, volume: 25, anno: 1994,
pagine: 200 - 208
SICI:
0169-328X(1994)25:3-4<200:DEOGRS>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
GAMMA-AMINOBUTYRIC-ACID; OPERATED CHLORIDE CHANNELS; SHORT SLEEP MICE; MESSENGER-RNAS; RAT-BRAIN; A RECEPTORS; GENETIC-DIFFERENCES; ALCOHOL ANTAGONIST; INVERSE AGONIST; ION CHANNELS;
Keywords:
GABA(A) RECEPTOR; ALPHA-1 SUBUNIT; ALPHA-6 SUBUNIT; BETA-2 SUBUNIT; WSR MOUSE; WSP MOUSE; ETHANOL; NORTHERN BLOT ANALYSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
51
Recensione:
Indirizzi per estratti:
Citazione:
W.J. Keir e A.L. Morrow, "DIFFERENTIAL EXPRESSION OF GABA(A) RECEPTOR SUBUNIT MESSENGER-RNAS INETHANOL-NAIVE WITHDRAWAL SEIZURE RESISTANT (WSR) VS WITHDRAWAL SEIZURE PRONE (WSP) MOUSE-BRAIN", Molecular brain research, 25(3-4), 1994, pp. 200-208

Abstract

Several lines of evidence suggest an important role for ethanol interactions with GABA(A) receptors in the development of the ethanol withdrawal syndrome. The present study was undertaken to determine whether there is a genetic relationship between ethanol withdrawal seizure severity and the expression of particular GABA(A) receptor subunits in mouse lines selectively bred for differential sensitivity to ethanol withdrawal seizures. Since GABA(A) receptor subunit levels are subject tomodulation by ethanol, the levels of GABA(A) receptor alpha 1, alpha 6 and beta 2 subunit mRNAs were measured in cerebellum while alpha 1 and beta 2 subunit levels were determined in cerebral cortex of ethanol-naive WSR and WSP mice. Poly(A)(+) RNA was isolated from groups of 6-10 animals and the GABA(A) receptor subunit mRNA levels were quantified by Northern blot analysis using subunit selective cRNA probes. In the cerebellum greater levels of each of these subunit mRNAs were detected in WSR1 mice compared to WSP1 mice. The levels of GABA(A) receptor alpha 1 subunit mRNAs were approximately 26 +/- 16 percent greater forthe 4.4 kb transcript and 84 +/- 23 percent greater for the 4.8 kb transcript in WSR mice vs WSP mice. GABA(A) receptor alpha 6 subunit (2.7 kb) mRNA levels in cerebellum were 159 +/- 58 percent greater in WSRmice than WSP mice, while beta 2 subunit mRNA levels were 110 +/- 30 percent greater in WSR than WSP mice. These results were replicated for the alpha 1 and alpha 6 subunits in WSR2 vs WSP2 mouse cerebella. Nodifferences in beta-actin mRNA levels were detected on the same RNA blots. Data normalized to beta-actin mRNA levels showed similar differences between WSR and WSP mice. In contrast, no differences in GABA(A) receptor alpha 1 or beta 2 subunit mRNA expression were detected in cerebral cortex between the WSR and WSP mouse lines. These data suggest that withdrawal seizure severity and the increased sensitivity to GABA(A) receptor antagonists in WSP vs. WSR mice may be related to differences in GABA(A) receptor gene expression in specific brain regions.

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Documento generato il 31/03/20 alle ore 10:20:53