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Titolo:
HEPATOCYTES EXHIBIT SUPERIOR TRANSGENE EXPRESSION AFTER TRANSPLANTATION INTO LIVER AND SPLEEN COMPARED WITH PERITONEAL-CAVITY OR DORSAL FATPAD - IMPLICATIONS FOR HEPATIC GENE-THERAPY
Autore:
GUPTA S; VEMURU RP; LEE CD; YERNENI PR; ARAGONA E; BURK RD;
Indirizzi:
YESHIVA UNIV ALBERT EINSTEIN COLL MED,MARION BESSIN LIVER RES CTR,ULLMAN 625,1300 MORRIS PK AVE BRONX NY 10461 YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT MED BRONX NY 10461 YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT PEDIAT BRONX NY 10461 YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT MICROBIOL & IMMUNOL BRONX NY 10461 YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT OBSTET & GYNECOL BRONX NY 10461
Titolo Testata:
Human gene therapy
fascicolo: 8, volume: 5, anno: 1994,
pagine: 959 - 967
SICI:
1043-0342(1994)5:8<959:HESTEA>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
MICROCARRIER-ATTACHED HEPATOCYTES; RAT HEPATOCYTES; GLUTAMINE-SYNTHETASE; SYNGENEIC HOSTS; FACTOR-I; B VIRUS; ANTIGEN; INDUCTION; MOUSE; CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
S. Gupta et al., "HEPATOCYTES EXHIBIT SUPERIOR TRANSGENE EXPRESSION AFTER TRANSPLANTATION INTO LIVER AND SPLEEN COMPARED WITH PERITONEAL-CAVITY OR DORSAL FATPAD - IMPLICATIONS FOR HEPATIC GENE-THERAPY", Human gene therapy, 5(8), 1994, pp. 959-967

Abstract

For hepatic gene therapy or applications of hepatocyte transplantation in liver failure, survival and function of transplanted cells is critical. Insights into site-specific gene regulation will significantly facilitate development of appropriate strategies for transplanting hepatocytes. To assess the function of transplanted cells, we used a transgenic hepatitis B virus (HBV) hepatocyte system, which allowed analysis of cellular gene expression with HBV surface antigen (HBsAg) mRNA expression, as well as secretion of HBsAg into peripheral circulation. When congeneic HBV hepatocytes were transplanted into the liver (via spleen), serum HBsAg promptly appeared in circulation and persisted forthe entire duration of the studies. In contrast, transplantation of hepatocytes into the peritoneal cavity or dorsal fat pad resulted in serum HBsAg levels that were either significantly lower or gradually rose after a lag period. HBsAg mRNA expression was several-fold greater in transplanted hepatocytes in liver or spleen versus in peritoneal cavity or dorsal fat pad. Despite persistence of transplanted hepatocytesin peritoneal cavity or dorsal fat pad, serum HBsAg was cleared by antibody to HBsAg (anti-HBs) but this was not observed after hepatocyte transplantation into spleen, As the function of transplanted hepatocytes is optimally regulated in the liver, hepatic reconstitution with cell transplantation will be most appropriate for gene therapy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/21 alle ore 23:42:12