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Titolo:
HIGH-MOBILITY-GROUP PROTEIN-1 AND PROTEIN-2 RECOGNIZE CHROMIUM-DAMAGED DNA
Autore:
WANG JF; BASHIR M; ENGELSBERG BN; WITMER C; ROZMIAREK H; BILLINGS PC;
Indirizzi:
UNIV PENN,SCH DENT MED,DEPT PATHOL PHILADELPHIA PA 19104 UNIV PENN,SCH DENT MED,DEPT PATHOL PHILADELPHIA PA 19104 UNIV PENN,SCH DENT MED,DEPT ANAT & HISTOL PHILADELPHIA PA 19104 RUTGERS STATE UNIV,ENVIRONM & OCCUPAT HLTH SCI INST PISCATAWAY NJ 08855 UNIV PENN,ANIM MED LAB PHILADELPHIA PA 19104
Titolo Testata:
Carcinogenesis
fascicolo: 2, volume: 18, anno: 1997,
pagine: 371 - 375
SICI:
0143-3334(1997)18:2<371:HPAPRC>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
CISPLATIN; BINDING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
J.F. Wang et al., "HIGH-MOBILITY-GROUP PROTEIN-1 AND PROTEIN-2 RECOGNIZE CHROMIUM-DAMAGED DNA", Carcinogenesis, 18(2), 1997, pp. 371-375

Abstract

Chromium (Cr) is a human carcinogen and a potent DNA damaging agent, Incubation of DNA with CrCl3 resulted in dose-dependent binding of Cr to DNA and, at concentrations >20 mu M, altered the electrophoretic mobility of a 100 bp oligonucleotide. We also demonstrate that high mobility group (HMG) proteins 1 and 2 bind Cr-damaged DNA (Cr-DNA). Protein binding was lesion density-dependent, with maximal binding to DNA treated with 100 mu M CrCl3. HMG2 binds to Cr-DNA with a calculated K-d Of similar to 10(-9) M. These proteins also bound DNA obtained from chromate-treated cells, These results suggest that the covalent attachment of Cr to DNA induces alterations in DNA structure which are recognized by HMG1 and HMG2 Therefore, these proteins may function as Cr-damaged DNA recognition proteins in vivo and as a consequence of binding, may play a role in directing the cellular response to Cr-DNA adduct formation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 18:28:13