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Titolo:
THE ONTOGENY OF INSULIN-LIKE GROWTH-FACTOR (IGF) AND IGF-BINDING PROTEIN GENE-EXPRESSION IN THE RAT PANCREAS
Autore:
HOGG J; HILL DJ; HAN VKM;
Indirizzi:
ST JOSEPHS HLTH CTR,LAWSON RES INST,MRC,FETAL & NEONATAL HLTH & DEV GRP,268 GROSVENOR ST LONDON N6A 4V2 ON CANADA ST JOSEPHS HLTH CTR,LAWSON RES INST,MRC,FETAL & NEONATAL HLTH & DEV GRP LONDON N6A 4V2 ON CANADA UNIV WESTERN ONTARIO,DEPT MED LONDON N6A 5A5 ON CANADA UNIV WESTERN ONTARIO,DEPT PAEDIAT LONDON N6A 5A5 ON CANADA UNIV WESTERN ONTARIO,DEPT BIOCHEM LONDON N6A 5A5 ON CANADA UNIV WESTERN ONTARIO,DEPT ANAT LONDON N6A 5A5 ON CANADA UNIV WESTERN ONTARIO,DEPT PHYSIOL LONDON N6A 5A5 ON CANADA
Titolo Testata:
Journal of molecular endocrinology
fascicolo: 1, volume: 13, anno: 1994,
pagine: 49 - 58
SICI:
0952-5041(1994)13:1<49:TOOIG(>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-FETAL PANCREAS; FACTOR-I; TISSUE DISTRIBUTION; MOLECULAR-CLONING; MESSENGER-RNA; SOMATOMEDIN-C; HORMONAL-REGULATION; DNA-REPLICATION; ISLETS; CULTURE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
J. Hogg et al., "THE ONTOGENY OF INSULIN-LIKE GROWTH-FACTOR (IGF) AND IGF-BINDING PROTEIN GENE-EXPRESSION IN THE RAT PANCREAS", Journal of molecular endocrinology, 13(1), 1994, pp. 49-58

Abstract

Insulin is important for optimal fetal and neonatal growth and development. Its continued availability is due, in part, to ongoing islet cell growth within the pancreas. IGFs and IGF-binding proteins (IGFBPs) have been implicated as paracrine regulators of islet cell growth within the developing pancreas. The purpose of this study was to determinewhether the intact rat pancreas expresses mRNAs for IGF-I, IGF-II andIGFBPs, and how these might change with development. Liver was studied as a control tissue. Pancreas and liver were taken from fetal rats at 20-22 days of gestation, from postnatal rats at 1-21 days and from adult animals, and mRNAs for IGFs-I and -II and IGFBPs-1 to -6 were detected by Northern blot hybridization. The amount of IGF-II mRNA was greatest in the liver and pancreas of the fetal rat, and declined in both tissues during the neonatal period. Conversely, IGF-I mRNA levels were low but detectable in fetal life, and rose to adult levels within 2weeks of birth. Both IGFBP-1 and IGFBP-2 mRNAs were present in fetal rat liver, increasing in amount over the first week of life, and declining in the adult. However, within the pancreas, IGFBP-1 mRNA was undetectable and IGFBP-2 mRNA was very low in the fetus and neonate. Both IGFBP-1 and IGFBP-2 mRNAs transiently appeared in the pancreas betweenpostnatal weeks 2 and 3 and declined in the adult. IGFBP-3 and IGFBP-4 mRNAs were detected in both the liver and pancreas throughout the developmental period studied. IGFBP-3 mRNA increased in amount immediately following birth, while the quantity of IGFBP-4 mRNA increased sharply in liver from postnatal day 21, but declined in the pancreas. mRNA for IGFBP-5 or -6 was undetectable in either tissue. The results show that both IGF-I and IGF-II are expressed by rat pancreas from at least20 days of gestation, the latter being predominant in fetal life and the former during postnatal development. In addition, at least four IGFBP mRNAs (IGFBPs-1, -2, -3 and -4) were expressed within the pancreaswith distinct developmental patterns. IGFBP-3 and -4 were predominantin the fetal and neonatal periods, while increased expression of IGFBPs-1 and -2 occurred 2-3 weeks after birth. The ontogeny of IGFBP mRNAexpression in pancreas differed from that in liver. The temporal and spatially specific pattern of IGF and IGFBP gene expression within thedeveloping pancreas supports a paracrine role for the IGF-IGFBP axis during pancreatic development in the rat.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 18:59:15