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Titolo:
VISUALIZATION AND QUANTIFICATION OF GLYCOLIPID POLARITY DYNAMICS IN THE PLASMA-MEMBRANE OF THE MAMMALIAN SPERMATOZOON
Autore:
GADELLA BM; GADELLA TWJ; COLENBRANDER B; VANGOLDE LMG; LOPESCARDOZO M;
Indirizzi:
AFRC,BABRAHAM INST,BABRAHAM HALL CAMBRIDGE CB2 4AT ENGLAND UNIV UTRECHT,VET BIOCHEM LAB 3508 TD UTRECHT NETHERLANDS UNIV UTRECHT,DEPT HERD HLTH & ANIM REPROD UTRECHT NETHERLANDS MAX PLANCK INST BIOPHYS CHEM W-3400 GOTTINGEN GERMANY
Titolo Testata:
Journal of Cell Science
, volume: 107, anno: 1994,
parte:, 8
pagine: 2151 - 2163
SICI:
0021-9533(1994)107:<2151:VAQOGP>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
GUINEA-PIG SPERM; DIFFERENTIAL SCANNING CALORIMETRY; MONOCLONAL-ANTIBODIES O1; ACROSOME REACTION; BOAR SPERMATOZOA; INVITRO CAPACITATION; SEMINAL PLASMA; EPIDIDYMAL SPERMATOZOA; IMMOBILIZING PROTEIN; PHASE-TRANSITIONS;
Keywords:
SEMINOLIPID; SPERM; PLASMA MEMBRANE; LIPID POLARITY; FLUORESCENCE LIFETIME IMAGING MICROSCOPY; IMMUNOLOCALIZATION; CAPACITATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
68
Recensione:
Indirizzi per estratti:
Citazione:
B.M. Gadella et al., "VISUALIZATION AND QUANTIFICATION OF GLYCOLIPID POLARITY DYNAMICS IN THE PLASMA-MEMBRANE OF THE MAMMALIAN SPERMATOZOON", Journal of Cell Science, 107, 1994, pp. 2151-2163

Abstract

Seminolipid (sulphogalactosylalkylacylglycerol), the glycolipid that is specific for mammalian germ cells, is located exclusively in the outer leaflet of the sperm plasma membrane. In this study the lateral distribution of seminolipid on sperm heads has been investigated by indirect immunofluorescence labelling and detection with digital imaging fluorescence microscopy. In freshly ejaculated sperm cells this glycolipid was present primarily at the apical ridge subdomain of the plasma membrane of the sperm head. After binding the sperm cells to zona-coated coverslips seminolipid migrated, in 40 minutes, from the apical ridge to the equatorial subdomain of the plasma membrane. A similar redistribution of seminolipid was observed during capacitation of sperm cells in vitro induced by Ca2+ or bovine serum albumin. Comparable migration of seminolipid was also found after prolonged storage of ejaculated sperm cells, albeit at a much slower rate. Addition of arylsulphatase A, an enzyme present in seminal plasma that desulphates seminolipid,significantly enhanced the migration of seminolipid during storage ofsperm cells. Its breakdown product desulphoseminolipid (galactosylalkylacylglycerol) appeared highly specifically at the equatorial segment. The measured fluorescence intensity over the sperm head surface correlated linearly with the spatial probe distribution as was checked by fluorescence lifetime imaging microscopy. This paper demonstrates and quantifies for the first time the polarity of seminolipid on the surface of the sperm cell and the dynamic alterations that occur in this polarity during post-ejaculatory events.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 04:03:40