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Titolo:
STABLE EXPRESSION OF HETEROLOGOUS MICROTUBULE-ASSOCIATED PROTEINS (MAPS) IN CHINESE-HAMSTER OVARY CELLS - EVIDENCE FOR DIFFERING ROLES OF MAPS IN MICROTUBULE ORGANIZATION
Autore:
BARLOW S; GONZALEZGARAY ML; WEST RR; OLMSTED JB; CABRAL F;
Indirizzi:
UNIV TEXAS,SCH MED,DEPT PHARMACOL,POB 20708 HOUSTON TX 77225 UNIV TEXAS,SCH MED,DEPT PHARMACOL HOUSTON TX 77225 UNIV ROCHESTER,DEPT BIOL ROCHESTER NY 14627
Titolo Testata:
The Journal of cell biology
fascicolo: 4, volume: 126, anno: 1994,
pagine: 1017 - 1029
SICI:
0021-9525(1994)126:4<1017:SEOHMP>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAMMALIAN-CELLS; ALPHA-TUBULIN; BETA-TUBULIN; GAMMA-TUBULIN; TAU-PROTEIN; DROSOPHILA-MELANOGASTER; ASPERGILLUS-NIDULANS; NONNEURONAL CELLS; SODIUM-BUTYRATE; BINDING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
64
Recensione:
Indirizzi per estratti:
Citazione:
S. Barlow et al., "STABLE EXPRESSION OF HETEROLOGOUS MICROTUBULE-ASSOCIATED PROTEINS (MAPS) IN CHINESE-HAMSTER OVARY CELLS - EVIDENCE FOR DIFFERING ROLES OF MAPS IN MICROTUBULE ORGANIZATION", The Journal of cell biology, 126(4), 1994, pp. 1017-1029

Abstract

To study the effects of microtubule-associated proteins (MAPs) on in vivo microtubule assembly, cDNAs containing the complete coding sequences of a Drosophila 205-kD heat stable MAP, human MAP 4, and human tauwere stably transfected into CHO cells. Constitutive expression of the transfected genes was low in most cases and had no obvious effects on the viability of the transfected cell lines. High levels of expression, as judged by Western blots, immunofluorescence, and Northern blots, could be induced by treating cells with sodium butyrate. High levelsof MAPs were maintained for at least 24-48 h after removal of the sodium butyrate. Immunofluorescence analysis indicated that all three MAPs bound to cellular microtubules, but only the transfected tau caused a rearrangement of microtubules into bundles. Despite high levels of expression of these exogenous MAPs and the bundling of microtubules in cells expressing tau, transfected cells had normal levels of assembledand unassembled tubulin. With the exception of the tau-induced bundles, microtubules in transfected cells showed the same sensitivity as control cells to microtubule depolymerization by Colcemid. Further, all three MAPs were ineffective in reversing the taxol-dependent phenotypeof a CHO mutant cell line. The absence of a quantitative effect of any of these heterologous proteins on the assembly of tubulin suggests that these MAPs may have different roles in vivo from those inferred previously from in vitro experiments.

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Documento generato il 12/07/20 alle ore 11:26:44