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Titolo:
IMMUNOCYTOCHEMICAL IDENTIFICATION OF THE SITE OF VITELLOGENIN SYNTHESIS IN THE FRESH-WATER PRAWN MACROBRACHIUM-NIPPONENSE
Autore:
HAN CH; OKUMURA T; SUZUKI Y; AIDA K; HANYU I;
Indirizzi:
DONGEUI UNIV,COLL NAT SCI,DEPT BIOL PUSAN 614714 SOUTH KOREA UNIV TOKYO,FAC AGR,DEPT FISHERIES,FISH PHYSIOL LAB,BUNKYO KU TOKYO 113 JAPAN
Titolo Testata:
Fisheries science
fascicolo: 2, volume: 60, anno: 1994,
pagine: 149 - 154
SICI:
0919-9268(1994)60:2<149:IIOTSO>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTIBODY ENZYME METHOD; FAT-BODY; ARMADILLIDIUM-VULGARE; PORCELLIO-DILATATUS; CRUSTACEAN ISOPODA; TERRESTRIAL ISOPOD; PENAEUS-VANNAMEI; CARCINUS-MAENAS; OVARY; VITELLIN;
Keywords:
PRAWN; VITELLOGENIN SYNTHESIS; LIPOVITELLIN; HEPATOPANCREAS; IMMUNOCYTOCHEMISTRY; MACROBRACHIUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
C.H. Han et al., "IMMUNOCYTOCHEMICAL IDENTIFICATION OF THE SITE OF VITELLOGENIN SYNTHESIS IN THE FRESH-WATER PRAWN MACROBRACHIUM-NIPPONENSE", Fisheries science, 60(2), 1994, pp. 149-154

Abstract

The site of vitellogenin synthesis in the freshwater prawn Macrobrachium nipponense was examined immunocytochemically using antibodies raised against lipovitellin purified from mature M. nipponense ovaries. The molecular weight of purified lipovitellin was estimated to be 350 kDa by gel filtration. The antiserum cross-reacted with vitellogenin in the hemolymph of the mature female prawn, and could therefore be used to demonstrate the possible site of vitellogenin synthesis immunocytochemically employing the peroxidase-anti-peroxidase (PAP) reaction. Hepatopancreas, sub-epidermal tissue, muscle, and ovary were fixed with periodate-lysine paraformaldehyde solution after which paraffin sections were made. In mature females, oocytes at the yolk globule stage, thehepatopancreas and the sub-epidermal tissue of the lateral somites showed positive PAP reactions. However, the sub-epidermal tissue of the ventral somites, follicular cells of the ovary and muscles did not. Nopositive reaction was observed in males and immature females. These results suggest that vitellogenin is synthesized in the hepatopancreas and at the sub-epidermal tissue in females at the exogenous vitellogenesis stage.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 18:31:25