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Titolo:
QUANTITATIVE PROTON SPECTROSCOPY OF CANINE BRAIN - IN-VIVO AND IN-VITRO CORRELATIONS
Autore:
BARKER PB; BREITER SN; SOHER BJ; CHATHAM JC; FORDER JR; SAMPHILIPO MA; MAGEE CA; ANDERSON JH;
Indirizzi:
217 TRAYLOR BLDG,720 RUTLAND AVE BALTIMORE MD 21205 JOHNS HOPKINS UNIV,SCH MED,DEPT RADIOL & RADIOL SCI BALTIMORE MD 00000
Titolo Testata:
Magnetic resonance in medicine
fascicolo: 2, volume: 32, anno: 1994,
pagine: 157 - 163
SICI:
0740-3194(1994)32:2<157:QPSOCB>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAGNETIC-RESONANCE SPECTROSCOPY; HUMAN VISUAL-CORTEX; L-ASPARTIC ACID; NMR-SPECTROSCOPY; ABSOLUTE QUANTITATION; H-1-NMR SPECTROSCOPY; WATER-CONTENT; METABOLITES; INVIVO; LACTATE;
Keywords:
QUANTITATION; PROTON NMR SPECTROSCOPY; CANINE BRAIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
56
Recensione:
Indirizzi per estratti:
Citazione:
P.B. Barker et al., "QUANTITATIVE PROTON SPECTROSCOPY OF CANINE BRAIN - IN-VIVO AND IN-VITRO CORRELATIONS", Magnetic resonance in medicine, 32(2), 1994, pp. 157-163

Abstract

Quantitative, single-voxel proton NMR spectroscopy of normal brain was performed in five adult beagle dogs using the cerebral water signal as an internal intensity reference. The same brain regions were then rapidly isolated and frozen using a pneumatic biopsy drill, perchloric acid extracted, and analyzed by biochemical assay and high-resolution NMR spectroscopy. The concentrations of the major resonances in the invivo and in vitro spectra were compared, and good agreement was foundbetween the different measurements. The in vivo spectra contained three peaks at 3.21, 3.04, and 2.02 ppm, which are usually assigned to trimethylamines (TMA), creatines, and N-acetyl derivatives (NAc), which corresponded to be the following metabolite concentration values: 1.7 /- 0.6, 7.7 +/- 2.1, and 10.9 +/- 2.7 mu mol/g wet weight respectively. In vitro, the following metabolite concentrations were measured: glycerophosphocholine (GPC) 1.3 +/- 0.2, phosphocholine (PC) 0.5 +/- 0.1, phosphocreatine (PCr) 2.6 +/- 0.4, creatine (Cr) 5.9 +/- 1.4, and N-Acetyl aspartate(NAA) 8.9 +/- 1.8 mu mol/g wet weight. Therefore, the 3.21 ppm resonance observed in the in vivo spectrum is predominantly GPC and PC in a ratio of 2.6:1, the 3.04 ppm resonance is Cr and PCr ina ratio of 2.3:1, and the 2.02 ppm resonance is predominantly (approximate to 80%) NAA with small contributions from N-acetylaspartylglutamate (NAAG) and glutamate. The data presented here validate the technique of water referencing as a simple and convenient means of quantitating single-voxel in vivo proton NMR spectra of the brain.

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Documento generato il 23/11/20 alle ore 20:19:06