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Titolo:
MODULATION OF GLYCINE AFFINITY FOR NMDA RECEPTORS BY EXTRACELLULAR CA2+ IN TRIGEMINAL NEURONS
Autore:
GU YP; HUANG LYM;
Indirizzi:
UNIV TEXAS,MED BRANCH,INST MARINE BIOMED,H43,200 UNIV BLVD GALVESTON TX 77555 UNIV TEXAS,MED BRANCH,INST MARINE BIOMED GALVESTON TX 77555 UNIV TEXAS,MED BRANCH,DEPT PHYSIOL & BIOPHYS GALVESTON TX 77555
Titolo Testata:
The Journal of neuroscience
fascicolo: 7, volume: 14, anno: 1994,
pagine: 4561 - 4570
SICI:
0270-6474(1994)14:7<4561:MOGAFN>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
METHYL-D-ASPARTATE; CULTURED HIPPOCAMPAL-NEURONS; LONG-TERM POTENTIATION; MOUSE CENTRAL NEURONS; DORSAL HORN NEURONS; PROTEIN-KINASE-C; VOLTAGE-DEPENDENT BLOCK; EXCITATORY AMINO-ACIDS; RAT CENTRAL NEURONS; CALCIUM PERMEABILITY;
Keywords:
NMDA RECEPTORS; GLUTAMATE RECEPTORS; ION CHANNELS; GLYCINE BINDING; CALCIUM; TRIGEMINAL NEURONS; DORSAL HORN NEURONS; NOCICEPTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
64
Recensione:
Indirizzi per estratti:
Citazione:
Y.P. Gu e L.Y.M. Huang, "MODULATION OF GLYCINE AFFINITY FOR NMDA RECEPTORS BY EXTRACELLULAR CA2+ IN TRIGEMINAL NEURONS", The Journal of neuroscience, 14(7), 1994, pp. 4561-4570

Abstract

Glycine and the divalent cation Ca2+ play key roles in regulating theactivity of excitatory amino acid NMDA receptor channels. There is accumulating evidence that the concentration of glycine at the synaptic cleft is below a saturated level. We examined the effect of external Ca2+ on NMDA responses in various concentrations of glycine in isolatedtrigeminal neurons. We found that external Ca2+ potentiated NMDA responses and this potentiation occurred only when glycine sites were unsaturated. Since single-channel conductance decreases in the high external Ca2+ solution, the observation cannot be explained by an increase in Ca2+ influx through the channels. Studying the dose-response curves for glycine in different Ca2+ solutions, we found that the apparent dissociation constant (EC(50)) for glycine decreases with increasing external Ca2+ concentrations. Kinetics studies of glycine binding to NMDAreceptors indicated that external Ca2+ causes a decrease in the off rate of the glycine binding, while having no effect on the on rate. Ouranalyses suggest that the apparent glycine affinity increases by about 3.7 times in Ca-containing solution. Thus, external Ca2+ contributesto the unusually high glycine affinity for NMDA receptors and may have a role in regulating the NMDA receptor channel activities during intensive or sustained neuronal stimulation.

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Documento generato il 07/07/20 alle ore 12:34:02