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Titolo:
EXPRESSION OF NON-NMDA GLUTAMATE-RECEPTOR CHANNEL GENES BY CLONAL HUMAN NEURONS
Autore:
HARDY M; YOUNKIN D; TANG CM; PLEASURE J; SHI QY; WILLIAMS M; PLEASURE D;
Indirizzi:
CHILDRENS HOSP PHILADELPHIA,DIV NEUROL RES PHILADELPHIA PA 19104 UNIV PENN,SCH MED,DEPT NEUROL PHILADELPHIA PA 19104 CHILDRENS HOSP PHILADELPHIA,DIV NEUROL RES PHILADELPHIA PA 19104
Titolo Testata:
Journal of neurochemistry
fascicolo: 2, volume: 63, anno: 1994,
pagine: 482 - 489
SICI:
0022-3042(1994)63:2<482:EONGCG>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXCITATORY POSTSYNAPTIC CURRENTS; RAT HIPPOCAMPAL-NEURONS; AMPA-KAINATE RECEPTORS; MOUSE CENTRAL NEURONS; D-ASPARTATE RECEPTORS; HUMAN CELL-LINE; FUNCTIONAL EXPRESSION; MOLECULAR-CLONING; STRUCTURAL DETERMINANTS; PROGENITOR-CELL;
Keywords:
GLUTAMATE RECEPTOR CHANNELS; EXCITOTOXICITY; NEURONS; KAINATE; PHA-AMINO-3-HYDROXY-5-METHYL-4-ISOXAZOLEPROPIONATE; PATCH CLAMP; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; CELL CULTURE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
80
Recensione:
Indirizzi per estratti:
Citazione:
M. Hardy et al., "EXPRESSION OF NON-NMDA GLUTAMATE-RECEPTOR CHANNEL GENES BY CLONAL HUMAN NEURONS", Journal of neurochemistry, 63(2), 1994, pp. 482-489

Abstract

Treatment of the human teratocarcinoma line NTera2/c1.D1 (NT2) with retinoic acid induces terminal neuronal differentiation. In a previous study, we found that the neurons obtained in this way express functional N-methyl-D-aspartate (NMDA) and non-NMDA glutamate receptor channels. We now show by reverse transcriptase-polymerase chain reaction and Southern blotting that these neurons transcribe each of the nine knownnon-NMDA glutamate receptor genes (GluR1-7, Ka-1, and Ka-2) and that four of these genes (GluR2, GluR6, GluR7, and Ka-1) are also transcribed by undifferentiated NT2 cells. Patch clamp studies demonstrate thatindividual non-NMDA glutamate receptor channels are readily isolated from NT2-derived neurons and that these channels are potently modulated by the desensitization blocker cyclothiazide. NT2-derived neurons are susceptible to kainate excitotoxicity but are not injured by prolonged exposure to ha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate. We expect that the NT2-derived human neuronal culture system will facilitate studies of human neuronal non-NMDA glutamate receptor channels and of the pathophysiology of neuronal excitotoxicity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 18:38:45