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Titolo:
MECHANISM OF CHOLINERGIC STIMULATION OF GLUCOSE-OXIDATION IN ISOLATEDGASTRIC GLANDS
Autore:
HERNANDEZ I; CHACIN J;
Indirizzi:
UNIV ZULIA,FAC MED,INST INVEST BIOL,INVEST GASTROINTESTINALES LAB,APARTADO 526 MARACAIBO VENEZUELA UNIV ZULIA,FAC MED,INST INVEST BIOL,INVEST GASTROINTESTINALES LAB MARACAIBO VENEZUELA
Titolo Testata:
The American journal of physiology
fascicolo: 2, volume: 267, anno: 1994,
parte:, 1
pagine: 70000227 - 70000234
SICI:
0002-9513(1994)267:2<70000227:MOCSOG>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
PYRUVATE-DEHYDROGENASE ACTIVITY; FREE CA-2+ CONCENTRATION; PARIETAL-CELLS; SECRETAGOGUE STIMULATION; ACID-SECRETION; RUTHENIUM RED; CALCIUM-IONS; MITOCHONDRIA; MUCOSA; METABOLISM;
Keywords:
PARIETAL CELL; OXIDATIVE METABOLISM; GASTRIC SECRETAGOGUES; CARBACHOL; PYRUVATE DEHYDROGENASE; OXOGLUTARATE DEHYDROGENASE; CALCIUM; IONOMYCIN; ,2-BIS(2-AMINOPHENOXY)ETHANE-N,N,N',N'-TETRAACETIC ACID ACETOXYMETHYLESTER; MITOCHONDRIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
I. Hernandez e J. Chacin, "MECHANISM OF CHOLINERGIC STIMULATION OF GLUCOSE-OXIDATION IN ISOLATEDGASTRIC GLANDS", The American journal of physiology, 267(2), 1994, pp. 70000227-70000234

Abstract

The mechanisms of cholinergic activation of carbohydrate metabolism were investigated in isolated rabbit gastric glands. Carbachol stimulated the rate of glucose oxidation in a dose-dependent fashion with a half-maximal effect occurring at similar to 9 mu M. Atropine and omeprazole, but not cimetidine, completely blocked the stimulation induced bycarbachol. Direct activation of the H+-K+ -adenosinetriphosphatase byNH4+ caused a significant stimulation of glucose oxidation that was totally abolished by oligomycin and by the mitochondrial uncouplers dinitrophenol and carbonyl cyanide p-trifluoromethoxyphenylhydrazone. These latter agents did not abolish the stimulating effect of carbachol on glucose oxidation. Ionomycin increased the rate of glucose oxidationin a dose-dependent manner, and this effect was not blocked by oligomycin. The metabolic effect of ionomycin was reduced but not abolished by omeprazole. 1,2-Bis(2-aminophenoxy)ethane-N,N,N' ,N'-tetraacetic acid acetoxymethyl ester eliminated the carbachol-induced stimulation ofglucose oxidation and partially inhibited the effect of NH4+. The mitochondrial enzymes pyruvate dehydrogenase and oxoglutarate dehydrogenase were activated by physiological concentrations of calcium in the isolated mitochondria. This effect was blocked by incubation with ruthenium red. The results suggest that calcium ions play a central role in the mechanism of cholinergic activation of carbohydrate oxidation in isolated rabbit gastric glands and that both indirect and direct actions on metabolism may be involved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 22:22:48