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Titolo:
HUMORAL AND CELL-MEDIATED-IMMUNITY IN RABBITS IMMUNIZED WITH LIVE NONREPLICATING AVIPOX RECOMBINANTS EXPRESSING THE HIV-1(SF2) ENV GENE
Autore:
RADAELLI A; GIMELLI M; CREMONESI C; SCARPINI C; MORGHEN CD;
Indirizzi:
UNIV MILAN,DEPT PHARMACOL,VIA VANVITELLI 32 I-20129 MILAN ITALY UNIV MILAN,DEPT PHARMACOL I-20129 MILAN ITALY UNIV MILAN,INST PHARMACOL SCI I-20122 MILAN ITALY UNIV MILAN,CNR,CTR CYTOPHARMACOL I-20122 MILAN ITALY
Titolo Testata:
Vaccine
fascicolo: 12, volume: 12, anno: 1994,
pagine: 1110 - 1117
SICI:
0264-410X(1994)12:12<1110:HACIRI>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; HUMAN MONOCLONAL-ANTIBODY; VACCINIA VIRUS; NEUTRALIZING ANTIBODIES; ENVELOPE GLYCOPROTEIN; SYNTHETIC PEPTIDE; ANIMAL-MODELS; TYPE-1 HIV-1; INFECTION; GP120;
Keywords:
AVIPOX-ENV RECOMBINANTS; HIV-1; IMMUNITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
51
Recensione:
Indirizzi per estratti:
Citazione:
A. Radaelli et al., "HUMORAL AND CELL-MEDIATED-IMMUNITY IN RABBITS IMMUNIZED WITH LIVE NONREPLICATING AVIPOX RECOMBINANTS EXPRESSING THE HIV-1(SF2) ENV GENE", Vaccine, 12(12), 1994, pp. 1110-1117

Abstract

The canarypox (CP) and fowl pox (FP) viruses, which are unable to replicate productively in non-avian species, have been utilized as live vectors carrying the HIV-1(SF2) env gene with the putative immunosuppressive (IS) region complete (CPIS+ and FPIS+) or deleted (CPIS- and FPIS-), To determine if these avipox-env recombinants could be utilized to elicit a specific immune response against HIV-1, six groups of rabbits were immunized with CPIS+, CPIS-, FPIS+, FPIS- constructs or their non-engineered wild-type CPwt or FPwt, counterparts. After a primary inoculation and successive boosters, env-specific humoral and cell-mediated immunity were demonstrated by ELISA, immunoblots and lymphoproliferation assays. Antibody titres and neutralization activities were higher in CP- than FP-inoculated rabbits, the CPIS+ always showing a similar immunogenic capacity to CPIS-. Evidence is also presented indicating that rabbit sera possess group-specific antibodies, which were, however, unable to cross-neutralize divergent HIV-1 strains. Although theprotective capacity against HIV-1 experimental infection has not yet been determined in these animals, our results suggest that these recombinants might represent promising and safer candidate vaccines againstHIV-1.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/10/20 alle ore 00:39:36