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Titolo:
POTENTIAL PROBLEMS IN USING [S-35] DATP-TAILED OLIGONUCLEOTIDES FOR DETECTING MESSENGER-RNAS IN CERTAIN CELLS OF THE IMMUNE-SYSTEM
Autore:
MEZEY E; HOFFMAN BJ; HARTA G; PALKOVITS M; NORTHUP J;
Indirizzi:
NINCDS,CLIN SCI LAB,BLDG 36,RM 3A17 BETHESDA MD 20892 NIMH,CELL BIOL LAB BETHESDA MD 20892 SEMMELWEIS UNIV MED,SCH MED,DEPT ANAT 1 H-1085 BUDAPEST HUNGARY
Titolo Testata:
The Journal of histochemistry and cytochemistry
fascicolo: 9, volume: 42, anno: 1994,
pagine: 1277 - 1283
SICI:
0022-1554(1994)42:9<1277:PPIU[D>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXPRESSION; CLONING; GENE;
Keywords:
SIGNAL AMPLIFICATION; S-35 LABELED OLIGONUCLEOTIDES; IN SITU HYBRIDIZATION HISTOCHEMISTRY; HISTAMINE H-2 RECEPTOR;
Tipo documento:
Note
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
16
Recensione:
Indirizzi per estratti:
Citazione:
E. Mezey et al., "POTENTIAL PROBLEMS IN USING [S-35] DATP-TAILED OLIGONUCLEOTIDES FOR DETECTING MESSENGER-RNAS IN CERTAIN CELLS OF THE IMMUNE-SYSTEM", The Journal of histochemistry and cytochemistry, 42(9), 1994, pp. 1277-1283

Abstract

In this study we examined the cause of unusually intense signals obtained in immune cells by in situ hybridization histochemistry using S-35-labeled oligonucleotides. We verified that the phenomenon is an amplification of a specific signal due to a series of chemical interactions after the probe binds to a specific mRNA in the tissue. The presenceof oxidative enzymes in the tissue seems to be necessary for this reaction to occur. Therefore, most cells of the immune system (e.g., macrophages, neutrophil and eosinophil leukocytes), being rich in oxidative enzymes, will show some signal amplification. The intensification ofthe signal can be avoided if MgCl2 is substituted for CoCl2 in the synthesis of [S-35]-thiophosphate-labeled probes, if 2,3-dimercaptopropanol [British anti-Lewisite (BAL)] is added to the hybridization buffer, or if [P-33]-phosphate is used instead of [S-35]-thiophosphate in the labeling of the probes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 08:22:32