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Titolo:
2 DISTINCT MECHANISMS REGULATE LUTEOVIRUS TRANSMISSION EFFICIENCY ANDSPECIFICITY AT THE APHID SALIVARY-GLAND
Autore:
PEIFFER ML; GILDOW FE; GRAY SM;
Indirizzi:
PENN STATE UNIV,DEPT PLANT PATHOL UNIVERSITY PK PA 16802 PENN STATE UNIV,DEPT PLANT PATHOL UNIVERSITY PK PA 16802 CORNELL UNIV,USDA ARS ITHACA NY 14853 CORNELL UNIV,DEPT PLANT PATHOL ITHACA NY 14853
Titolo Testata:
Journal of General Virology
, volume: 78, anno: 1997,
parte:, 3
pagine: 495 - 503
SICI:
0022-1317(1997)78:<495:2DMRLT>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
BARLEY YELLOW DWARF; BASAL LAMINAE; RECEPTOR; MOSQUITO; BINDING; INSECT; VIRUS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
M.L. Peiffer et al., "2 DISTINCT MECHANISMS REGULATE LUTEOVIRUS TRANSMISSION EFFICIENCY ANDSPECIFICITY AT THE APHID SALIVARY-GLAND", Journal of General Virology, 78, 1997, pp. 495-503

Abstract

Barley yellow dwarf luteovirus (BYDV) particles are transmitted by aphids in a species-specific manner. Transmission to plants requires that the virus particles be transported across the basal lamina and plasmalemma of the accessory salivary gland (ASG). To characterize the roleof the ASG basal lamina in regulating BYDV transmission, five aphid species were microinjected with purified New York isolates BYDV-PAV or -RPV, Both viruses associated specifically only with the ASG basal lamina, The ability of virions to penetrate the basal lamina was separatefrom the ability to penetrate the plasmalemma. When the salivary glands of vector, Sitobion avenae or non-vector, Rhopalosiphum maidis, aphids were incubated in vitro with New York isolate BYDV-MAV, virions only attached to the ASG basal lamina of S. avenae. When anionic and cationic ferritin were microinjected into aphids, only cationic ferritin aggregated on the surface of the ASG basal lamina and at openings of plasmalemma invaginations into the cytoplasm, suggesting that these sites had a net negative charge. In vitro studies of anionic and cationicgold penetration of ASG basal laminae indicated a macromolecular sizeexclusion limit of approximately 20 nm that depended on charge, Anionic gold particles did not accumulate in the basal lamina as densely asthe 25 nm BYDV particles, suggesting that the virus particles have a greater affinity for the ASG basal lamina. These results indicate thatboth the ASG basal lamina and plasmalemma contain specific componentsindependently involved in the recognition and transmission of luteoviruses.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 03:34:12