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Titolo:
ISOLATION AND CHARACTERIZATION OF LONG-TERM EMBRYOGENIC LINES IN ASPARAGUS-OFFICINALIS L
Autore:
DELBREIL B; GOEBELTOURAND I; LEFRANCOIS C; JULLIEN M;
Indirizzi:
UNIV SCI & TECH LILLE FLANDRES ARTOIS,PHYSIOL VEGETALE LAB F-59655 VILLENEUVE DASCQ FRANCE INRA,BIOL CELLULAIRE LAB F-78026 VERSAILLES FRANCE JACQUES MARIONNET GFA,IN VITRO LAB F-41230 SOINGS EN SOLOGNE FRANCE UNIV PARIS 06,PHYSIOL DEV PLANTES LAB F-75252 PARIS 05 FRANCE
Titolo Testata:
Journal of plant physiology
fascicolo: 2, volume: 144, anno: 1994,
pagine: 194 - 200
SICI:
0176-1617(1994)144:2<194:IACOLE>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
HORMONE-FREE MEDIUM; SOMATIC EMBRYOGENESIS; SUSPENSION-CULTURES; REGENERATION; CELLS; PROPAGATION; REQUIREMENT; PLANTS; CARROT; SHOOT;
Keywords:
ASPARAGUS OFFICINALIS L; HABITUATION; SOMATIC EMBRYOGENESIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
B. Delbreil et al., "ISOLATION AND CHARACTERIZATION OF LONG-TERM EMBRYOGENIC LINES IN ASPARAGUS-OFFICINALIS L", Journal of plant physiology, 144(2), 1994, pp. 194-200

Abstract

Somatic embryogenesis was obtained from 14 Asparagus officinalis L. genotypes by in vitro culture of different explant types such as cladophylls, apices and isolated mesophyll cells. Explants first induced on a culture medium containing growth regulators produced calli and developed few somatic embryos when transferred on a hormone-free medium. During this development phase, we isolated a new type of friable tissuesthat produced abundant somatic embryos. The frequencies of emergence of these highly embryogenic tissues were estimated between 10(-5) and 10(-6). These tissue were habituated; being established as long-term embryogenic lines by repeated subcultures on hormone-free medium, continuously they produced numerous somatic embryos, some of them converting to whole plants. Histological data showed these long-term embryogenic lines to grow by recurrent embryogenesis arising, from single epidermal cells of preexisting embryos.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 19:42:28