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Titolo:
IDENTIFICATION OF 4 METABOLITES OF 3-(PHENYLAMINO)ALANINE, A CONSTITUENT IN L-TRYPTOPHAN PRODUCTS IMPLICATED IN EOSINOPHILIA-MYALGIA-SYNDROME, IN RATS
Autore:
ADACHI J; MIO T; UENO Y; NAITO T; NISHIMURA A; FUJIWARA S; SUMINO K; TATSUNO Y;
Indirizzi:
KOBE UNIV,SCH MED,DEPT LEGAL MED KOBE 650 JAPAN KOBE UNIV,SCH MED,DEPT PUBL HLTH KOBE 650 JAPAN KOBE WOMENS COLL PHARM KOBE 658 JAPAN
Titolo Testata:
Archives of toxicology
fascicolo: 8, volume: 68, anno: 1994,
pagine: 500 - 505
SICI:
0340-5761(1994)68:8<500:IO4MO3>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
PEAK-E; ACID; EMS; SUBSTANCE; SAMPLES;
Keywords:
3-(PHENYLAMINO)ALANINE; L-TRYPTOPHAN; EOSINOPHILIA-MYALGIA SYNDROME; RAT URINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
12
Recensione:
Indirizzi per estratti:
Citazione:
J. Adachi et al., "IDENTIFICATION OF 4 METABOLITES OF 3-(PHENYLAMINO)ALANINE, A CONSTITUENT IN L-TRYPTOPHAN PRODUCTS IMPLICATED IN EOSINOPHILIA-MYALGIA-SYNDROME, IN RATS", Archives of toxicology, 68(8), 1994, pp. 500-505

Abstract

3-(Phenylamino)alanine (PAA), a contaminant found in L-tryptophan tablets, has been discussed as a possible cause of eosinophilia-myalgia syndrome (EMS). We administered PAA (100 mg/kg) by gastric gavage to Wistar rats to determine its distribution and metabolism. We developed apurification procedure, using Bond Elut SCX cartridges followed by high performance liquid chromatography (HPLC) in order to determine levels of PAA. The level of PAA in blood was 4.22 mu g/ml at 5 h and urinary excretion was 21.7 mu g for 5 h and 84.6 mu g between 5 and 24 h. The amount of PAA in the contents of the large intestine at 5 h was 0.76 mu g, indicating poor transfer of PAA to the large intestine. However, the highest concentration of PAA was 12.3 mu g/g in the brain, indicating the passage of PAA through the blood-brain barrier. In additionto detecting PAA in the blood and organs, we also detected four metabolites of PAA in urine. We used gas chromatography mass spectrometry to identify PAA in rat liver, as well as N-(hydroxyphenyl)glycine, N-phenylglycine, 3-(pheylamino)lactic acid, and 3-(hydroxyphenylamino)lactic acid in rat urine. These results suggest that the degradation pathway of PAA is similar to that of phenylalanine.

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Documento generato il 07/08/20 alle ore 00:10:28