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Titolo:
3-WAY AND 2-WAY REARRANGEMENTS INVOLVING CHROMOSOMES 10, 2, 5 AND 5, 2 IN 2 MARKER CHROMOSOMES OF A HUMAN-MELANOMA CELL-LINE
Autore:
DONEDA L; WIEGANT J; LARIZZA L;
Indirizzi:
UNIV MILAN,FAC MED,DEPT BIOL & GENET,VIA VIOTTI 3-5 I-20133 MILAN ITALY UNIV MILAN,FAC MED,DEPT BIOL & GENET,VIA VIOTTI 3-5 I-20133 MILAN ITALY
Titolo Testata:
Melanoma research
fascicolo: 4, volume: 4, anno: 1994,
pagine: 259 - 265
SICI:
0960-8931(1994)4:4<259:3A2RIC>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Keywords:
AMPLIFICATION REARRANGEMENT; FLUORESCENCE IN-SITU HYBRIDIZATION; HETEROCHROMATIN; MARKER CHROMOSOMES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
NO
Recensione:
Indirizzi per estratti:
Citazione:
L. Doneda et al., "3-WAY AND 2-WAY REARRANGEMENTS INVOLVING CHROMOSOMES 10, 2, 5 AND 5, 2 IN 2 MARKER CHROMOSOMES OF A HUMAN-MELANOMA CELL-LINE", Melanoma research, 4(4), 1994, pp. 259-265

Abstract

Two marker chromosomes (marl and mar2), provided with two closely spaced heterochromatic bands, were observed in the 14932 cell line established from a human metastatic melanoma. Fluorescence in situ hybridization (FISH) with the alphoid sequence p82H common to all human centromeres showed strong signals over the double C-bands of mar1 and mar2. These were recognized by a chromosome 2-specific alphoid probe, although chromosome in situ suppression (CISS) hybridization with a chromosome 2 library failed to reveal any painting along marl and mar2. The centromere of mar1 was identified by a chromosome 10-specific alphoid sequence and the marker chromosome was decorated from pter to a region proximal to the interpolated C-band by a chromosome 10 library. The centromere of mar2 could not be recognized by any chromosome-specific alphoid probe, but the whole mar2 was decorated by a chromosome 5 library. This library also painted the distal q arm of mar1, which was not painted by the chromosome 10 library, as well as a small band proximal tothe double C-band. Identification of the two marker chromosomes reveals their common origin and indicates a role for chromosomes 2, 5 and 10 in the genesis and/or progression of the 14932 melanoma. Alteration to the chromosome-specific alphoid sequence in the centromere of mar2 provides evidence for rearrangement of constitutive heterochromatin alphoid sequences in human tumours.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 15:19:06