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Titolo:
DIAGNOSIS OF CUTANEOUS LEISHMANIASIS AND SPECIES DISCRIMINATION OF PARASITES BY PCR AND HYBRIDIZATION
Autore:
RODRIGUEZ N; GUZMAN B; RODAS A; TAKIFF H; BLOOM BR; CONVIT J;
Indirizzi:
IVIC,INST BIOMED CARACAS VENEZUELA IVIC,CTR MICROBIOL CARACAS VENEZUELA YESHIVA UNIV ALBERT EINSTEIN COLL MED,HOWARD HUGHES MED INST BRONX NY10461
Titolo Testata:
Journal of clinical microbiology
fascicolo: 9, volume: 32, anno: 1994,
pagine: 2246 - 2252
SICI:
0095-1137(1994)32:9<2246:DOCLAS>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
KINETOPLAST DNA MINICIRCLES; IDENTIFICATION; AMPLIFICATION; BRAZILIENSIS; GUATEMALA; SEQUENCES; COLOMBIA; WORLD;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
N. Rodriguez et al., "DIAGNOSIS OF CUTANEOUS LEISHMANIASIS AND SPECIES DISCRIMINATION OF PARASITES BY PCR AND HYBRIDIZATION", Journal of clinical microbiology, 32(9), 1994, pp. 2246-2252

Abstract

The aim of this study was to assess the efficacy of PCR methodology in establishing the diagnosis of cutaneous leishmaniasis in patients from areas of endemicity in Venezuela. Biopsies from 233 patients with cutaneous ulcers suggestive of leishmaniasis were analyzed by PCR, employing oligonucleotides directed against conserved regions of kinetoplast DNA (kDNA), and the PCR products were then hybridized to nonradioactively labeled, species-specific, cloned kDNA fragments. The ability of PCR to detect Leishmania cells was compared with those of the conventional methodologies: skin testing with killed promastigotes (Montenegro test), examination of Giemsa-stained biopsy smears, and in vitro culture of biopsy tissue. The PCR-hybridization technique detected the presence of Leishmania cells in 98% of patients clinically diagnosed ashaving leishmaniasis and also positive by the Montenegro skin test. In comparison, leishmania positivity was found in only 42% of cultures and 64% of biopsy smears. By hybridizing the PCR product to new kDNA probes specific for either Leishmania mexicana or Leishmania braziliensis we found that both species are major causes of cutaneous leishmaniasis in Venezuela, and the species identification was confirmed by restriction enzyme analysis of kDNA from biopsy cultures. This work demonstrates that PCR coupled with hybridization is useful not only for the diagnosis of cutaneous leishmaniasis but also for the taxonomic discrimination essential for both epidemiology and therapy. This technique can be used to diagnose leishmaniasis in a country in which the diseaseis endemic and can perhaps be adapted for use in a rural clinic.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 19:18:06