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Titolo:
SELF-AGGREGATION OF THE TRANSMEMBRANE GLYCOPROTEIN CD38 PURIFIED FROMHUMAN ERYTHROCYTES
Autore:
FRANCO L; ZOCCHI E; CALDER L; GUIDA L; BENATTI U; DEFLORA A;
Indirizzi:
UNIV GENOA,INST BIOCHEM,VIALE BENEDETTO XV-1 I-16132 GENOA ITALY UNIV GENOA,INST BIOCHEM I-16132 GENOA ITALY ADV BIOTECHNOL CTR I-16132 GENOA ITALY NATL INST MED RES,DIV VIROL LONDON NW7 1AA ENGLAND
Titolo Testata:
Biochemical and biophysical research communications
fascicolo: 3, volume: 202, anno: 1994,
pagine: 1710 - 1715
SICI:
0006-291X(1994)202:3<1710:SOTTGC>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYCLIC ADP-RIBOSE; RECEPTOR DIMERIZATION; OUTER SURFACE; CELLS; PROTEINS; HYDROLYSIS; BINDING; NAD+;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
L. Franco et al., "SELF-AGGREGATION OF THE TRANSMEMBRANE GLYCOPROTEIN CD38 PURIFIED FROMHUMAN ERYTHROCYTES", Biochemical and biophysical research communications, 202(3), 1994, pp. 1710-1715

Abstract

The 46 kDa human transmembrane glycoprotein CD38 is a multicatalytic enzyme exhibiting ADPribosyl cyclase, cyclic ADPribose hydrolase and NAD(+)-glycohydrolase activities at its extracellular domain. When CD38, purified to homogeneity from human erythrocyte membranes, was incubated with NAD(+) or beta-mercaptoethanol, extensive aggregation took place. Addition of both compounds to CD38 led to the formation of still larger aggregates (over 300 nm), which were resistant to TCA precipitation. Extensive and stable CD38 self-aggregation was shown by, i) SDS-PAGE and autoradiography of the [P-32]NAD(+)-incubated CD38, ii) SDS-PAGE followed by immunochemical detection of CD38 on the transblots, iii) direct electron microscopy on negatively stained CD38 samples. Self-aggregation of CD38 might be correlated with its putative function asa transducer of activation and proliferation signals in a number of hematopoietic cells. (C) 1994 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/10/20 alle ore 10:01:54