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Titolo:
CHARACTERIZATION OF THE BOVINE MONOCYTE CHEMOATTRACTANT PROTEIN-1 GENE
Autore:
WEMPE F; KUHLMANN JK; SCHEIT KH;
Indirizzi:
MAX PLANCK INST BIOPHYS CHEM,DEPT MOLEC BIOL D-37077 GOTTINGEN GERMANY MAX PLANCK INST BIOPHYS CHEM,DEPT MOLEC BIOL D-37077 GOTTINGEN GERMANY
Titolo Testata:
Biochemical and biophysical research communications
fascicolo: 3, volume: 202, anno: 1994,
pagine: 1272 - 1279
SICI:
0006-291X(1994)202:3<1272:COTBMC>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLOOD MONONUCLEAR LEUKOCYTES; CDNA CLONING; MOLECULAR-CLONING; CYTOKINE FAMILY; SPLEEN-CELLS; EXPRESSION; SEQUENCE; BINDING; MCP-1; TRANSCRIPTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
F. Wempe et al., "CHARACTERIZATION OF THE BOVINE MONOCYTE CHEMOATTRACTANT PROTEIN-1 GENE", Biochemical and biophysical research communications, 202(3), 1994, pp. 1272-1279

Abstract

Bovine monocyte chemoattractant protein-1 (bovine MCP-1) cDNA has recently been characterized and shown to be highly expressed in bovine seminal vesicles. In an attempt to isolate the MCP-1 gene, we screened abovine genomic lambda EMBL-3 library with an MCP-1 specific probe pH42. A positive clone lambda MCP1 was subjected to restriction analysis and a pH42-positive EcoRI-fragment of 3.8 kb was subcloned and sequenced. The bovine MCP-1 gene consists of three exons separated by two introns. We undertook to identify homologous sequences 5'-upstream of thetranscriptional start site within the genes of rat, mouse, human and bovine MCP-1. Six conserved sequence stretches located within 400 bp upstream of the transcriptional start site in MCP-I genes were detected. Interestingly an approximately 45 bp long region, displaying identities in the range of 68-72% relative to the human sequence, covers in the rat gene the -141/-88 region responsible for TPA induction of gene expression. (C) 1994 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 22:10:22