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Titolo:
IMPROVED OPERATIONAL STABILITY OF CELL-FREE GLUCOSE-FRUCTOSE OXIDOREDUCTASE FROM ZYMOMONAS-MOBILIS FOR THE EFFICIENT SYNTHESIS OF SORBITOL AND GLUCONIC ACID IN A CONTINUOUS ULTRAFILTRATION MEMBRANE REACTOR
Autore:
NIDETZKY B; FURLINGER M; GOLLHOFER D; SCOPES RK; HALTRICH D; KULBE KD;
Indirizzi:
AGR UNIV VIENNA,INST FOOD TECHNOL,MUTHGASSE 18 A-1190 VIENNA AUSTRIA LA TROBE UNIV,CTR PROT & ENZYME TECHNOL BUNDOORA VIC 3083 AUSTRALIA
Titolo Testata:
Biotechnology and bioengineering
fascicolo: 6, volume: 53, anno: 1997,
pagine: 623 - 629
SICI:
0006-3592(1997)53:6<623:IOSOCG>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERMEABILIZED CELLS; ENZYME; LOCALIZATION; REGENERATION;
Keywords:
GLUCOSE-FRUCTOSE OXIDOREDUCTASE; ZYMOMONAS MOBILIS; FREE ENZYME; CONTINUOUS PRODUCTION; STABILITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
B. Nidetzky et al., "IMPROVED OPERATIONAL STABILITY OF CELL-FREE GLUCOSE-FRUCTOSE OXIDOREDUCTASE FROM ZYMOMONAS-MOBILIS FOR THE EFFICIENT SYNTHESIS OF SORBITOL AND GLUCONIC ACID IN A CONTINUOUS ULTRAFILTRATION MEMBRANE REACTOR", Biotechnology and bioengineering, 53(6), 1997, pp. 623-629

Abstract

For the continuous, enzymatic synthesis of sorbitol and gluconic acidby cell-free glucose-fructose oxidoreductase (GFOR) from Zymomonas mobilis, the principal determinants of productivity have been identified. Most important, the rapid inactivation of the soluble enzyme during substrate conversion can be avoided almost completely when weak bases such as tris(hydroxymethyl) aminomethan or imidazol are used for the titration of the produced gluconic acid and when 5-10 mM dithiothreitolare added to prevent thiol oxidations. With regard to a long-term operational stability of the enzyme for continuous syntheses, thermal deactivation becomes significant at reaction temperatures above 30 degrees C. Without any additional purification being required, the crude cell extract of Z. mobilis can be employed in a continuous ultrafiltration membrane reactor over a time period of more than 250 h without significant decrease in substrate conversion or enzyme activity. The use ofsoluble GFOR thus appears to be an interesting alternative to employing permeabilized cells of Zymomonas for the production of sorbitol andgluconic acid and may be superior with regard to reactor productivities, at comparable operational stabilities. (C) 1997 John Wiley & Sons,Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 06:34:18