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Titolo:
ANALYSIS OF GLYCOPROTEINS SEPARATED BY 2-DIMENSIONAL GEL-ELECTROPHORESIS USING LECTIN BLOTTING REVEALED BY CHEMILUMINESCENCE
Autore:
GRAVEL P; GOLAZ O; WALZER C; HOCHSTRASSER DF; TURLER H; BALANT LP;
Indirizzi:
PSYCHIAT UNIV INST GENEVA,CLIN RES UNIT,CHEMIN PETIT BEL AIR 2 CH-1225 CHENE BOURG SWITZERLAND UNIV HOSP GENEVA,DEPT INTERNAL MED GENEVA SWITZERLAND UNIV GENEVA,DEPT MOLEC BIOL CH-1211 GENEVA SWITZERLAND
Titolo Testata:
Analytical biochemistry
fascicolo: 1, volume: 221, anno: 1994,
pagine: 66 - 71
SICI:
0003-2697(1994)221:1<66:AOGSB2>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYACRYLAMIDE GELS; OLIGOSACCHARIDE CHAINS; NITROCELLULOSE SHEETS; PROTEINS; BLOTS; PEROXIDASE; RESOLUTION; MEMBRANES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
P. Gravel et al., "ANALYSIS OF GLYCOPROTEINS SEPARATED BY 2-DIMENSIONAL GEL-ELECTROPHORESIS USING LECTIN BLOTTING REVEALED BY CHEMILUMINESCENCE", Analytical biochemistry, 221(1), 1994, pp. 66-71

Abstract

The carbohydrate structures of blotted glycoproteins can be analyzed by probing with lectins. The objective of the present work was to optimize the lectin blotting of human plasma glycoproteins separated by two-dimensional gel electrophoresis and the detection by the sensitive chemiluminescence method. The proposed detection method was found to beten times more sensitive than a standard colorimetric reaction. Furthermore, the generated signals are detected on a X-ray film and providea permanent record. The method is also very reliable when compared tothe colorimetric detection. The present procedure for glycoprotein analysis is particularly well suited for screening changes in glycosylation of proteins in biological samples. (C) 1994 Academic Press,Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 11:42:21