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Titolo:
COOPERATIVE BENDING OF THE 21-BASE-PAIR REPEATS OF THE SV40 VIRAL EARLY PROMOTER BY HUMAN SP1
Autore:
SUN D; HURLEY LH;
Indirizzi:
UNIV TEXAS,COLL PHARM,INST DRUG DYNAM AUSTIN TX 78712
Titolo Testata:
Biochemistry
fascicolo: 32, volume: 33, anno: 1994,
pagine: 9578 - 9587
SICI:
0006-2960(1994)33:32<9578:CBOT2R>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
EUKARYOTIC TRANSCRIPTIONAL ACTIVATORS; ANTITUMOR ANTIBIOTIC CC-1065; DNA-SEQUENCE SPECIFICITY; SYNERGISTIC ACTIVATION; GEL-ELECTROPHORESIS; BINDING; (+)-CC-1065; PROTEIN; INHIBITION; COMPLEXES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
53
Recensione:
Indirizzi per estratti:
Citazione:
D. Sun e L.H. Hurley, "COOPERATIVE BENDING OF THE 21-BASE-PAIR REPEATS OF THE SV40 VIRAL EARLY PROMOTER BY HUMAN SP1", Biochemistry, 33(32), 1994, pp. 9578-9587

Abstract

The overall structural features of the multimeric complex between Sp1and the 21-base-pair repeat of the early promoter region of SV40 DNA have been determined using hydroxyl-radical footprinting; (+)-CC-1065,a sequence-specific minor groove bending probe; and circularization experiments. The results show that the 21-base-pair repeat region has an intrinsically in-phase bent structure that is stabilized upon saturation Sp1 binding by protein-DNA and protein-protein interactions to produce a looping structure. The direction of the Sp1-stabilized bendingof DNA occurs into the minor groove and is localized between each of the Sp1 binding sites. These results are used as the basis to propose a looping structure for the multimeric Sp1 21-base-pair repeat region of SV40 DNA. Last, these results provide a rationale for the recently observed inhibition of basal transcriptional levels by site-specific triple-helical DNA complexes.

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Documento generato il 28/11/20 alle ore 22:01:27