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Titolo:
DNASE-I FOOTPRINTING OF TRIPLE-HELIX FORMATION AT POLYPURINE TRACTS BY ACRIDINE-LINKED OLIGOPYRIMIDINES - STRINGENCY, STRUCTURAL-CHANGES AND INTERACTION WITH MINOR-GROOVE BINDING LIGANDS
Autore:
STONEHOUSE TJ; FOX KR;
Indirizzi:
UNIV SOUTHAMPTON,DEPT PHYSIOL & PHARMACOL,BASSETT CRESCENT E SOUTHAMPTON SO9 3TU HANTS ENGLAND UNIV SOUTHAMPTON,DEPT PHYSIOL & PHARMACOL SOUTHAMPTON SO9 3TU HANTS ENGLAND
Titolo Testata:
Biochimica et biophysica acta, N. Gene structure and expression
fascicolo: 3, volume: 1218, anno: 1994,
pagine: 322 - 330
SICI:
0167-4781(1994)1218:3<322:DFOTFA>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
FORMING OLIGONUCLEOTIDE; SEQUENCE; INHIBITION; CLEAVAGE; OLIGODEOXYNUCLEOTIDES; RECOGNITION; COMPLEX; STRAND; DRUG; SITE;
Keywords:
TRIPLE HELIX; YYR TRIPLER; DISTAMYCIN; MITHRAMYCIN; DNAASE FOOTPRINTING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
T.J. Stonehouse e K.R. Fox, "DNASE-I FOOTPRINTING OF TRIPLE-HELIX FORMATION AT POLYPURINE TRACTS BY ACRIDINE-LINKED OLIGOPYRIMIDINES - STRINGENCY, STRUCTURAL-CHANGES AND INTERACTION WITH MINOR-GROOVE BINDING LIGANDS", Biochimica et biophysica acta, N. Gene structure and expression, 1218(3), 1994, pp. 322-330

Abstract

We have investigated the binding of short (10 base) acridine-linked tripler-forming oligonucleotides to the target sequence A(6)G(6).C6T6 by DNase I footprinting. Specific binding is detected at low pH(<6.0) for 5'-Acr-T5C5 and 5'-ACr-(U5C5)-U-5Br-C-5Me.The sequence T5C5, lacking the acridine modification, binds less strongly, though specific binding is still evident. 5'-Acr-T5C5- produces footprints at slightly lower concentrations than 5'-Acr-(U5C5)-U-5Br-C-5Me. All three oligonucleotides produce enhanced DNase I digestion at the 3'-end of the target purine strand, suggesting that there is a DNA structural change at thetripler-duplex boundary. Target sequences A(n)G(4)A and TAC(3)T(n), containing one and two tripler mismatches, show no interaction with theacridine-free oligonucleotide, but bind the acridine-linked oligonucleotides. In these secondary binding modes the third strand is positioned so that the mismatches are located at the 3'-end of the oligonucleotide. Mithramycin and distamycin, binding in the minor groove to GC- and AT-rich sequences respectively, abolish triple helix formation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 15:38:44