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Titolo:
CIS-ACTING REGULATORY SEQUENCES GOVERNING WNT-1 EXPRESSION IN THE DEVELOPING MOUSE CNS
Autore:
ECHELARD Y; VASSILEVA G; MCMAHON AP;
Indirizzi:
HARVARD UNIV,BIOL LABS,DEPT CELLULAR & DEV BIOL,16 DIVIN AVE CAMBRIDGE MA 02138 ROCHE INST MOLEC BIOL,ROCHE RES CTR NUTLEY NJ 07110
Titolo Testata:
Development
fascicolo: 8, volume: 120, anno: 1994,
pagine: 2213 - 2224
SICI:
0950-1991(1994)120:8<2213:CRSGWE>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAMMARY ONCOGENE INT-1; EMBRYONAL CARCINOMA-CELLS; SEGMENT-POLARITY GENE; NEURAL CREST CELLS; PROTO-ONCOGENE; TRANSGENIC MICE; NEUROECTODERMAL DIFFERENTIATION; NUCLEOTIDE-SEQUENCE; DROSOPHILA EMBRYO; WINGLESS PROTEIN;
Keywords:
WNT-1-LACZ FUSIONS; TRANSGENIC MICE; MIDBRAIN; NEURAL CREST;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
69
Recensione:
Indirizzi per estratti:
Citazione:
Y. Echelard et al., "CIS-ACTING REGULATORY SEQUENCES GOVERNING WNT-1 EXPRESSION IN THE DEVELOPING MOUSE CNS", Development, 120(8), 1994, pp. 2213-2224

Abstract

The protooncogene Wnt-1 encodes a short-range signal which is first expressed in, and appears to demarcate, the presumptive midbrain, Absence of Wnt-1 expression leads to the loss of this region of the brain. By the end of neural tube closure, expression of Wnt-1 extends down much of the dorsal midline of the central nervous system (CNS). Expression is exclusively limited to the CNS at this and later stages. We haveinvestigated the regulation of Wnt-1 during mouse development. Analysis of the embryonic expression of Wnt-1-lacZ reporter constructs spanning nearly 30 kb of the Wnt-1 locus identified a 5.5 kb cis-acting 3' enhancer element which confers correct temporal and spatial expressionon the lacZ gene. Interestingly embryos express Wnt-1-lacZ transgenesin migrating neural crest cells which are derived from the dorsal CNS. Ectopic expression of the Wnt-1-lacZ transgenes mag result from perdurance of beta-galactosidase activity in migrating neural crest cells originating from a Wnt-1-expressing region of the dorsal CNS. Alternatively, ectopic expression may arise from transient de novo activation of the transgenes in this cell population. These results are a first step towards addressing how regional cell signaling is established in the mammalian CNS. In addition, transgene expression provides a new tool for the analysis of neural crest development in normal and mutant mouse embryos.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 21:54:23