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Titolo:
FUNCTIONS INVOLVED IN BACTERIOPHAGE-P2-INDUCED HOST-CELL LYSIS AND IDENTIFICATION OF A NEW TAIL GENE
Autore:
ZIERMANN R; BARTLETT B; CALENDAR R; CHRISTIE GE;
Indirizzi:
BOX 980678 RICHMOND VA 23298 UNIV CALIF BERKELEY,DEPT MOLEC & CELL BIOL BERKELEY CA 94720 VIRGINIA COMMONWEALTH UNIV,DEPT MICROBIOL & IMMUNOL RICHMOND VA 23298
Titolo Testata:
Journal of bacteriology
fascicolo: 16, volume: 176, anno: 1994,
pagine: 4974 - 4984
SICI:
0021-9193(1994)176:16<4974:FIIBHL>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
F-PLASMID GENE; ESCHERICHIA-COLI; NUCLEOTIDE-SEQUENCE; SATELLITE BACTERIOPHAGE-P4; PROMOTES DEGRADATION; DIRECTED MUTAGENESIS; LAMBDA-S; PROTEIN; ACID; DNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
54
Recensione:
Indirizzi per estratti:
Citazione:
R. Ziermann et al., "FUNCTIONS INVOLVED IN BACTERIOPHAGE-P2-INDUCED HOST-CELL LYSIS AND IDENTIFICATION OF A NEW TAIL GENE", Journal of bacteriology, 176(16), 1994, pp. 4974-4984

Abstract

Successful completion of the bacteriophage P2 lytic cycle requires phage-induced lysis of its Escherichia coli host, a process that is poorly understood. Genetic analysis of lysis-deficient mutants defined a single locus, gene X, which lies within the largest late transcription unit of P2 and maps between head gene L and tail gene R. We determinedand analyzed the DNA sequence of a ca. 2.1-kb EcoRV fragment that spans the entire region from L to R, thus completing the sequence of thisoperon. This region contains all of the functions necessary for host cell lysis. Sequence analysis revealed five open reading frames, initially designated orf19 through orf23. All of the existing lysis mutants-ts60, am12, am76, and am218-were located in oral, which must therefore correspond to gene K. The K gene product has extensive amino acid sequence similarity to the product of gene R of bacteriophage lambda, and it exhibits endolysin function. Site-directed mutagenesis and reverse genetics were used to create P2 amber mutants in each of the four other newly identified open reading frames. Both orf19 (gene X) and orf20 (gene Y) encode essential functions, whereas orf22 (lysA) and orf23 (lysB) are nonessential. Gene Y encodes a polypeptide with striking similarities to the family of holin proteins exemplified by gpS of phagelambda, and the Yam mutant displayed the expected properties of a holin mutant, The gene products of lysA and lysB, although nonessential, appear to play a role in the correct timing of lysis, since a lysA amber mutant caused slightly accelerated lysis and a lysB amber mutant slightly delayed lysis of nonpermissive strains. Gene X must encode a tail protein, since lysates from nonpermissive cells infected with the Xamber mutant were complemented in vitro by similar lysates of cells infected with P2 head mutants but not with tail mutants.

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Documento generato il 03/12/20 alle ore 15:13:28