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Titolo:
ESTABLISHMENT AND CHARACTERIZATION OF 4 HUMAN EPITHELIAL OVARIAN-CARCINOMA CELL-LINES
Autore:
HIRTE HW; KAISER JS; BACCHETTI S;
Indirizzi:
HAMILTON REG CANC CTR,ONTARIO CANC TREATMENT & RES FDN,DIV MED ONCOL,699 CONCESS ST HAMILTON L8V 5C2 ON CANADA HAMILTON REG CANC CTR,ONTARIO CANC TREATMENT & RES FDN,DIV GYNECOL ONCOL HAMILTON L8V 1C3 ON CANADA MCMASTER UNIV,DEPT MED HAMILTON ON CANADA MCMASTER UNIV,DEPT PATHOL HAMILTON ON CANADA
Titolo Testata:
Cancer
fascicolo: 3, volume: 74, anno: 1994,
pagine: 900 - 906
SICI:
0008-543X(1994)74:3<900:EACO4H>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
MUCINOUS CYSTADENOCARCINOMA; MONOCLONAL-ANTIBODIES; GROWTH-STIMULATION; INITIAL EVALUATION; CIS-PLATINUM; NUDE-MICE; INVITRO; CANCER; ADENOCARCINOMA; ANTIGENS;
Keywords:
OVARIAN NEOPLASM; CELL LINE; SERUM-FREE MEDIUM; ANCHORAGE-INDEPENDENT GROWTH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
H.W. Hirte et al., "ESTABLISHMENT AND CHARACTERIZATION OF 4 HUMAN EPITHELIAL OVARIAN-CARCINOMA CELL-LINES", Cancer, 74(3), 1994, pp. 900-906

Abstract

Background. To develop in vitro models of human ovarian carcinoma, fresh tumour cells derived from malignant effusions were cultured in vitro in the presence or absence of serum to establish cell lines. Methods. Cell lines established were characterized by morphology in culture,surface marker expression, cytogenetic analysis, and growth in anchorage-dependent and anchorage-independent conditions. Results. Four celllines (MAC-2, RIC-2, SCHM-1, and SIB-1) were established from tumor cells isolated from the malignant effusions of four patients with epithelial ovarian carcinoma. These lines were able to grow in the presenceof low concentrations of serum (3%), and two lines grew in the absence of serum (MAC-2 and SIB-1). All cell lines have been grown continuously for longer than 6 months. One cell line (SCHM-1) grew only in liquid medium whereas the other lines grew in both liquid and semi-solid media. Chromosome analysis revealed aneuploidy in three of the four lines. All of the lines stained positively for CA-125 and HMFG-2, consistent with an epithelial origin. Conclusion. The ability of these cells to grow in low concentrations of serum or in serum-free conditions should prove useful for the in vitro study of factors affecting the growth of human ovarian carcinoma. The serum-free medium developed will be of use in the isolation of factors from the conditioned medium of these cell lines and previously established cell lines.

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Documento generato il 29/11/20 alle ore 17:48:51