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Titolo:
DIMETHYL-SULFOXIDE MODULATES NF-KAPPA-B AND CYTOKINE ACTIVATION IN LIPOPOLYSACCHARIDE-TREATED MURINE MACROPHAGES
Autore:
KELLY KA; HILL MR; YOUKHANA K; WANKER F; GIMBLE JM;
Indirizzi:
OKLAHOMA MED RES FDN,IMMUNOBIOL & CANC PROGRAM,825 NE 13TH ST OKLAHOMA CITY OK 73104 OKLAHOMA MED RES FDN,IMMUNOBIOL & CANC PROGRAM OKLAHOMA CITY OK 73104 UNIV OKLAHOMA,HLTH SCI CTR,COLL DENT,DEPT ORTHODONT OKLAHOMA CITY OK 73190 UNIV OKLAHOMA,HLTH SCI CTR,COLL ALLIED HLTH SCI,DEPT CLIN LAB SCI OKLAHOMA CITY OK 73190 UNIV OKLAHOMA,HLTH SCI CTR,COLL MED,DEPT IMMUNOL & MICROBIOL OKLAHOMACITY OK 73190 UNIV OKLAHOMA,HLTH SCI CTR,COLL MED,DEPT PATHOL OKLAHOMA CITY OK 73190 OKLAHOMA CHRISTIAN UNIV,DEPT NAT SCI OKLAHOMA CITY OK 73136
Titolo Testata:
Infection and immunity
fascicolo: 8, volume: 62, anno: 1994,
pagine: 3122 - 3128
SICI:
0019-9567(1994)62:8<3122:DMNACA>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; MANGANOUS SUPEROXIDE-DISMUTASE; HUMAN-IMMUNODEFICIENCY-VIRUS; FACTOR-ALPHA GENE; TRANSCRIPTION FACTOR; CELL-LINES; BACTERIAL LIPOPOLYSACCHARIDES; INTERLEUKIN-6 GENE; T-CELL; ENDOTOXIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
58
Recensione:
Indirizzi per estratti:
Citazione:
K.A. Kelly et al., "DIMETHYL-SULFOXIDE MODULATES NF-KAPPA-B AND CYTOKINE ACTIVATION IN LIPOPOLYSACCHARIDE-TREATED MURINE MACROPHAGES", Infection and immunity, 62(8), 1994, pp. 3122-3128

Abstract

Antioxidants are protective against septic shock in animal models. Recently, free radical scavengers have been found to inhibit the activation of the NF-kappa B protein in a number of cell lines. This transcriptional regulatory protein binds to the promoters of the proinflammatory cytokines tumor necrosis factor, interleukin-6, and the macrophage inflammatory proteins. The current work examined lipopolysaccharide-induced NF-kappa B activation in the J774 macrophage-like cell line and primary peritoneal macrophages from lipopolysaccharide-responsive (C3HeB/Fej) and -nonresponsive (C3H/HeJ) murine strains. The DNA-binding activity of the NF-kappa B protein directly correlated with mRNA expression for the genes encoding the proinflammatory cytokines and the freeradical scavenging enzyme, superoxide dismutase. Both the p50 and p65NF-kappa B subunits were detected on gel supershift assays. Minimal NF-kappa B activity was observed following exposure of C3H/HeJ macrophages to lipopolysaccharide. The antioxidant dimethyl sulfoxide decreased the level of NF-kappa B activation in the J774 cells. This correlated with decreased expression of cytokine mRNAs and tumor necrosis factor bioactivity. These results suggest that modulation of NF-kappa B activation may provide a mechanism through which antioxidants protect against endotoxemia in murine models.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 15:31:57