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Titolo:
THE CHICKEN MX-PROMOTER CONTAINS AN ISRE MOTIF AND CONFERS INTERFERONINDUCIBILITY TO A REPORTER GENE IN CHICK AND MONKEY CELLS
Autore:
SCHUMACHER B; BERNASCONI D; SCHULTZ U; STAEHELI P;
Indirizzi:
UNIV FREIBURG,INST MIKROBIOL & HYG,VIROL ABT,HERMANN HERDER STR 11 D-79008 FREIBURG GERMANY UNIV FREIBURG,INST MIKROBIOL & HYG,VIROL ABT D-79008 FREIBURG GERMANY
Titolo Testata:
Virology
fascicolo: 1, volume: 203, anno: 1994,
pagine: 144 - 148
SICI:
0042-6822(1994)203:1<144:TCMCAI>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEQUENCE-BINDING-PROTEIN; TRANSCRIPTION FACTOR; ALPHA-INTERFERON; VIRUS; ELEMENT; MURINE; COMMON; FAMILY; ISGF-3; GAMMA;
Tipo documento:
Note
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
B. Schumacher et al., "THE CHICKEN MX-PROMOTER CONTAINS AN ISRE MOTIF AND CONFERS INTERFERONINDUCIBILITY TO A REPORTER GENE IN CHICK AND MONKEY CELLS", Virology, 203(1), 1994, pp. 144-148

Abstract

Genomic DNA containing the first four exons and upstream sequences ofthe interferon (IFN)-inducible chicken Mr gene was cloned and sequenced. The exon/intron structure of the chicken Mr gene resembles that ofthe mouse Mx1 gene: exon 1 is very small, the first intron contains anoncoding exon 1' that is alternatively spliced in different chicken breeds and exon 2 harbors the ATG initiation codon. Exons 3 and 4 of the 'chicken and mouse Mx genes have identical lengths and the encoded polypeptides show a high degree of homology. Sequences upstream of thetranscription start site functioned as an IFN-inducible promoter in chick and monkey cells when cloned in front of a promoterless luciferase reporter gene. Deletion analysis suggested that the sequence 5'AGTTTCGTTTCT3' is of critical importance for inducibility. It conforms to the consensus sequence of the IFN-stimulated response element (ISRE) present in Mr and other IFN-alpha/beta-inducible promoters of mammals. These results suggest that the ISRE motif has been conserved and that it regulates expression of IFN-inducible genes in both mammals and birds. (C) 1994 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 16:07:42