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Titolo:
QUALITY AND POSITION OF THE 3 LAC OPERATORS OF ESCHERICHIA-COLI DEFINE EFFICIENCY OF REPRESSION
Autore:
OEHLER S; AMOUYAL M; KOLKHOF P; VONWILCKENBERGMANN B; MULLERHILL B;
Indirizzi:
UNIV COLOGNE,INST GENET,WEYERTAL 121 D-50931 COLOGNE GERMANY UNIV COLOGNE,INST GENET D-50931 COLOGNE GERMANY INST COCHIN GENET MOLEC,INSERM,U129,UNITE RECH GENET & PATHOL MOLEC F-75014 PARIS FRANCE
Titolo Testata:
EMBO journal
fascicolo: 14, volume: 13, anno: 1994,
pagine: 3348 - 3355
SICI:
0261-4189(1994)13:14<3348:QAPOT3>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-DNA RECOGNITION; ESCHERICHIA-COLI; LAMBDA-REPRESSOR; LACTOSE OPERON; LOOP FORMATION; PROMOTER; BINDING; SITES; SEQUENCES; TRANSCRIPTION;
Keywords:
COOPERATIVITY; DNA LOOPS; LAC OPERATOR; LAC REPRESSOR; LOCAL CONCENTRATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
50
Recensione:
Indirizzi per estratti:
Citazione:
S. Oehler et al., "QUALITY AND POSITION OF THE 3 LAC OPERATORS OF ESCHERICHIA-COLI DEFINE EFFICIENCY OF REPRESSION", EMBO journal, 13(14), 1994, pp. 3348-3355

Abstract

Repression of the lac promoter may be achieved in two different ways:either by interference with the action of RNA polymerase or by interference with CAP activation. We investigated cooperative repression of the Escherichia coil lac operon by systematic conversion of its three natural operators (O1, O2 and O3) on the chromosome. We find that cooperative repression by tetrameric Lac repressor increases with both quality and proximity of the interacting operators. A short distance of 92 bp allows effective repression by two very weak operators (O3, O3). The cooperativity of lac operators is discussed in terms of a local increase of repressor concentration. This increase in concentration depends on flexible DNA which allows loop formation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 20:07:19