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Titolo:
THE EPSTEIN-BARR-VIRUS NUCLEAR ANTIGEN-2 TRANSACTIVATOR IS DIRECTED TO RESPONSE ELEMENTS BY THE J-KAPPA RECOMBINATION SIGNAL BINDING-PROTEIN
Autore:
GROSSMAN SR; JOHANNSEN E; TONG X; YALAMANCHILI R; KIEFF E;
Indirizzi:
HARVARD UNIV,SCH MED,DEPT MED,75 FRANCIS ST BOSTON MA 02115 HARVARD UNIV,SCH MED,DEPT MED BOSTON MA 02115 HARVARD UNIV,SCH MED,DEPT MICROBIOL & MOLEC GENET BOSTON MA 02115
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 16, volume: 91, anno: 1994,
pagine: 7568 - 7572
SICI:
0027-8424(1994)91:16<7568:TENATI>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN LYMPHOCYTES-B; RBP-J-KAPPA; TRANSCRIPTIONAL ACTIVATOR; GENE; DNA; SEQUENCE; EXPRESSION; DROSOPHILA; TRANSFORMATION; HAIRLESS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
S.R. Grossman et al., "THE EPSTEIN-BARR-VIRUS NUCLEAR ANTIGEN-2 TRANSACTIVATOR IS DIRECTED TO RESPONSE ELEMENTS BY THE J-KAPPA RECOMBINATION SIGNAL BINDING-PROTEIN", Proceedings of the National Academy of Sciences of the United Statesof America, 91(16), 1994, pp. 7568-7572

Abstract

Epstein-Barr virus nuclear antigen 2 (EBNA-2) plays an essential rolein primary B-lymphocyte growth transformation. EBNA-2 is an acidic transcriptional transactivator that is brought to virus and cell EBNA-2 response elements by interaction with a factor that recognizes the double-stranded sequence MNYYGTGGGAA, where M is A or C, N is any nucleotide, and Y is a pyrimidine. A 63-kDa protein that recognizes this DNA sequence has now been purified by S-Sepharose and oligonucleotide affinity chromatography. p63 peptide sequence is identical to the predicted amino acid sequence for the human J kappa immunoglobulin recombination signal binding protein. Purified or recombinant in vitro-translatedJ kappa binds to the MNYYGTGGGAA EBNA-2 response element sequence andinteracts with EBNA-2. Surprisingly, J kappa does not bind to the J(kappa)1 heptamer recombination signal sequence (CACTGTG), and its prioridentification as a heptamer binding protein was most likely due to the addition of a BamHI restriction site to the native heptamer creating a near EBNA-2 response element consensus (CACTGTGGGAT).

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Documento generato il 01/10/20 alle ore 00:14:56